Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 76945..77681 Replicon plasmid pKp_SB612_2
Accession NZ_CP084832
Organism Klebsiella pneumoniae strain SB612

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag LGM16_RS27280 Protein ID WP_003026803.1
Coordinates 76945..77427 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag LGM16_RS27285 Protein ID WP_003026799.1
Coordinates 77415..77681 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM16_RS27260 (LGM16_27260) 72664..73059 - 396 WP_004143398.1 helix-turn-helix domain-containing protein -
LGM16_RS27265 (LGM16_27265) 73108..74454 - 1347 WP_077253535.1 ISNCY family transposase -
LGM16_RS27270 (LGM16_27270) 74680..75312 + 633 WP_001567369.1 hypothetical protein -
LGM16_RS27275 (LGM16_27275) 75341..76744 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
LGM16_RS27280 (LGM16_27280) 76945..77427 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
LGM16_RS27285 (LGM16_27285) 77415..77681 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
LGM16_RS27290 (LGM16_27290) 78126..78356 - 231 WP_004206660.1 hypothetical protein -
LGM16_RS27295 (LGM16_27295) 78370..78573 - 204 WP_004213596.1 HHA domain-containing protein -
LGM16_RS27300 (LGM16_27300) 78634..79125 - 492 WP_004206662.1 hypothetical protein -
LGM16_RS27305 (LGM16_27305) 79259..79411 - 153 WP_223168441.1 hypothetical protein -
LGM16_RS27310 (LGM16_27310) 79509..80213 - 705 WP_136048995.1 IS6-like element IS26 family transposase -
LGM16_RS27315 (LGM16_27315) 80278..80592 - 315 Protein_83 transposase -
LGM16_RS27320 (LGM16_27320) 80962..81666 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
LGM16_RS27325 (LGM16_27325) 81729..81836 - 108 Protein_85 type-F conjugative transfer system secretin TraK -
LGM16_RS27330 (LGM16_27330) 81823..82389 - 567 WP_004144423.1 type IV conjugative transfer system protein TraE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..131535 131535
- inside IScluster/Tn - - 70994..81666 10672


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T219532 WP_003026803.1 NZ_CP084832:c77427-76945 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T219532 NZ_CP116490:c1014765-1014475 [Haemophilus influenzae]
ATGACTTATAAACTCATTTTTGATAAACGCGCATTAAAAGAATGGAATAAATTGGGGGAAACCTTACGCCAACAATTTAA
AAATAAATTAGCCGAAAGAATGATAAATCCACGCATTCAAGCCGACAAATTAAAGGGCGAAAATGATTTATATAAAATCA
AATTAAGAAGCGCTGGTTATCGATTAGTTTATCAAGTAAGAGATCAGGAAATCACTATTATTGTTGTATCTGTTGGAAAA
AGGGAACGATTAGAAGTCTATAAAACCGCAGAACAAAGAACAGCCCACTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT219532 WP_003026799.1 NZ_CP084832:c77681-77415 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT219532 NZ_CP116490:c1015009-1014755 [Haemophilus influenzae]
ATGGCATTACATCAAATACTGGCAGAACAAGCGGCAAGCATTACAGACTTAAAACGAAACCCAATGGGCGTAATTCAAGA
AAGCGAAAGCGGAATAGTCGCAATATTGAACCGAAACCAACCCGCATTTTATTGCATCACCCCCGAATTATTCTCTCATA
TGCAAGAATTAATCGAAGATTTAGAGCTCGGCAGAATGGCAAATGAACGGCTAGCCACCCTAGAACCAGTAGAGGTTGAT
CTAAATGACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References