Detailed information of TA system TA021953

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 3535968..3537611
• Replicon: chromosome
• Accession: NC_008825
• Organism: Methylibium petroleiphilum PM1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A2SL44
• Locus tag: MPE_RS16810
• Protein ID: WP_011830904.1
• Coordinates: 3535968..3537320 (-)
• Length: 451 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: MPE_RS16815
• Protein ID: WP_011830905.1
• Coordinates: 3537327..3537611 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MPE_RS16790	3531024..3532088	-	1065	WP_202796718.1	aldehyde dehydrogenase family protein	-
MPE_RS16795	3532143..3533156	+	1014	WP_011830032.1	IS5 family transposase	-
MPE_RS16800	3533276..3533698	-	423	WP_011830902.1	aldehyde dehydrogenase family protein	-
MPE_RS22870	3533906..3535960	+	2055	WP_011830903.1	EAL domain-containing protein	-
MPE_RS16810	3535968..3537320	-	1353	WP_011830904.1	type II toxin-antitoxin system HipA family toxin	Toxin
MPE_RS16815	3537327..3537611	-	285	WP_011830905.1	helix-turn-helix domain-containing protein	Antitoxin
MPE_RS16825	3537979..3539370	+	1392	WP_041929735.1	class II fumarate hydratase	-
MPE_RS16830	3539374..3540402	-	1029	WP_011830907.1	heat-inducible transcriptional repressor HrcA	-
MPE_RS16835	3540489..3541394	+	906	WP_011830908.1	NAD kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (10-110)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-67)

Sequences

Toxin

Protein

• Length: 451 a.a.
• Molecular weight: 49534.60 Da
• Isoelectric Point: 8.4933

>T21953 WP_011830904.1 NC_008825:c3537320-3535968 [Methylibium petroleiphilum PM1]
MGRRSHTRTLALWMNGERVGAWSTSPAGDELRYDAGWLRSPQVRPLSLSLPFRPGNPPHKGNAVRDYFENLLPDSRPIRE
RLARRFKTATTDAFDLLTEIGRDCVGALQILPDGQDPGDVKTVQARPLDEAGVARALNAALTPSGFDQRTADADEFRISI
AGAQEKTALLWHEGRWWRPMGPTPTTHILKLPLGLVGNMKLDLRDSVENEWLCAQVLEAYGLPVARCHPLAFEGQRVLGV
ERFDRMWADDGAWIIRLPQEDMCQATGTPAHAKYEADGGPGIDGILEVLNASRRRVQDRENFFRAQLMFWLMCAPDGHAK
NFSIALHAGGAFSMTPLYDVVSAYPVLGNGLNRISPHRVKLAMALRCTNAHWRMRDILRRHWEAVGRRSGIVSASGQGVD
GLIQDIIDRTPVALDAVAAKLPPGFPAYVADSILGGIRAAVGRLEAAKAS

Nucleotide

• Length: 1353 bp

>T21953 NC_008825:c3537320-3535968 [Methylibium petroleiphilum PM1]
ATGGGAAGGCGATCGCACACGCGCACGCTCGCTTTGTGGATGAACGGCGAGCGGGTGGGGGCCTGGAGCACCTCGCCCGC
CGGAGACGAGTTGCGCTACGACGCGGGCTGGTTGCGCTCGCCGCAGGTGAGGCCGCTGTCCCTGTCATTGCCCTTCAGGC
CGGGCAATCCGCCGCACAAGGGCAACGCCGTCCGCGACTACTTCGAGAACCTGCTGCCGGACAGCCGACCGATCCGTGAA
CGTCTGGCACGCCGGTTCAAGACAGCGACCACAGACGCCTTCGACCTGCTGACCGAGATCGGCCGCGATTGCGTCGGTGC
GCTGCAGATCCTGCCCGACGGGCAGGACCCCGGCGATGTGAAGACCGTCCAGGCCCGGCCGCTCGACGAGGCGGGTGTGG
CGCGGGCGCTGAACGCCGCGCTCACGCCCAGCGGCTTCGACCAACGAACCGCGGACGCGGACGAGTTCCGCATCTCGATC
GCCGGTGCACAGGAGAAGACGGCGCTGCTGTGGCACGAGGGTCGGTGGTGGCGTCCGATGGGACCGACACCCACCACCCA
CATCCTGAAGTTGCCGCTGGGCCTGGTGGGGAACATGAAGCTCGACCTGCGCGACTCGGTGGAGAACGAATGGCTGTGCG
CCCAGGTGCTGGAGGCCTACGGCCTGCCCGTCGCGCGCTGCCACCCGCTGGCGTTCGAGGGCCAGCGCGTCCTCGGCGTC
GAGCGTTTCGACCGGATGTGGGCAGACGATGGCGCCTGGATCATTCGCCTGCCGCAGGAAGACATGTGCCAGGCCACCGG
CACGCCGGCACACGCCAAATACGAGGCCGACGGCGGCCCGGGCATCGACGGCATTCTGGAGGTGCTGAACGCCTCGCGTC
GTCGCGTTCAGGACCGTGAGAACTTCTTCCGGGCCCAGCTGATGTTCTGGCTGATGTGCGCGCCGGACGGGCATGCCAAG
AACTTCAGCATCGCGCTGCACGCGGGCGGTGCCTTCTCGATGACGCCGCTCTACGACGTGGTGTCCGCCTATCCCGTGCT
GGGCAACGGCCTCAACCGGATTTCTCCGCATCGCGTCAAGCTGGCGATGGCGCTGCGGTGCACCAACGCGCACTGGCGCA
TGCGCGACATCCTGCGCCGGCATTGGGAGGCGGTCGGGCGGCGGAGCGGGATCGTGTCCGCGTCGGGGCAGGGCGTGGAC
GGGTTGATCCAGGACATCATCGACAGGACCCCCGTCGCCCTGGACGCAGTGGCCGCGAAGCTGCCGCCGGGCTTTCCGGC
CTACGTCGCCGATTCGATCCTGGGCGGCATCCGGGCCGCCGTGGGCCGGCTCGAAGCGGCGAAGGCCTCGTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 9874.32 Da
• Isoelectric Point: 10.7473

>AT21953 WP_011830905.1 NC_008825:c3537611-3537327 [Methylibium petroleiphilum PM1]
MDEFPVRTADQLPGLLQSFRKAAGLTQADLAARLGVTQQTLSALERNAGTVSAARLMKLLSILDVELVLRQQRSSGAASP
KAGGVPGGSEKAQW

Nucleotide

• Length: 285 bp

>AT21953 NC_008825:c3537611-3537327 [Methylibium petroleiphilum PM1]
ATGGACGAGTTCCCGGTGCGCACGGCAGACCAGCTTCCCGGCCTGCTGCAGAGCTTTCGCAAGGCGGCCGGCCTGACGCA
GGCAGACCTTGCCGCACGGCTCGGGGTCACGCAGCAGACGCTGTCTGCGCTCGAGCGCAATGCAGGCACCGTCAGCGCGG
CCCGGCTGATGAAGCTGCTCAGCATCCTCGATGTCGAGCTCGTGCTGAGGCAACAGCGGTCTTCGGGAGCTGCTTCACCC
AAGGCGGGCGGCGTACCCGGCGGGTCCGAAAAGGCGCAGTGGTAG

Structures

Toxin

• AlphaFold DB: A2SL44