Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5246849..5247474 Replicon chromosome
Accession NZ_CP084830
Organism Klebsiella pneumoniae strain SB612

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag LGM16_RS25560 Protein ID WP_019705794.1
Coordinates 5246849..5247232 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LGM16_RS25565 Protein ID WP_004150355.1
Coordinates 5247232..5247474 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM16_RS25545 (LGM16_25545) 5244215..5245117 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LGM16_RS25550 (LGM16_25550) 5245114..5245749 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LGM16_RS25555 (LGM16_25555) 5245746..5246675 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LGM16_RS25560 (LGM16_25560) 5246849..5247232 - 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
LGM16_RS25565 (LGM16_25565) 5247232..5247474 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LGM16_RS25570 (LGM16_25570) 5247679..5248596 + 918 WP_019705795.1 alpha/beta hydrolase -
LGM16_RS25575 (LGM16_25575) 5248610..5249551 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LGM16_RS25580 (LGM16_25580) 5249596..5250033 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LGM16_RS25585 (LGM16_25585) 5250030..5250890 - 861 WP_004146232.1 virulence factor BrkB family protein -
LGM16_RS25590 (LGM16_25590) 5250884..5251483 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T219529 WP_019705794.1 NZ_CP084830:c5247232-5246849 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T219529 NZ_CP116490:c527031-526894 [Haemophilus influenzae]
ATGTACGCGATTGCTTTTGATTTAGTTGTAAAAGACACTCAAGATTATCATCCAAAGGGCGTTCAAGAGGCTTATACAGA
CATTCGCGCTTTTCGTATTGAGCAATGGTCTGATTTTACTGATTTTATACGAAATTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT219529 WP_004150355.1 NZ_CP084830:c5247474-5247232 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT219529 NZ_CP116490:c527231-527040 [Haemophilus influenzae]
ATGGAACTAAGACAACAAATTCCAACAGGCTGTATTAAGCAATTTGGGCAATTTGGCGTGCCTTATGTGGTTGGCGAGGT
TGCGGAATTTTTGCCAGATGGTGATGTGTTGGTGAATATCACATTGTTACAATCTGGTGAAAAAGACATTTATCGCTTAT
CTCACCTACTTGAAGATCCCGAAGCTGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References