Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4658175..4658985 Replicon chromosome
Accession NZ_CP084830
Organism Klebsiella pneumoniae strain SB612

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LGM16_RS22755 Protein ID WP_004178461.1
Coordinates 4658175..4658708 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LGM16_RS22760 Protein ID WP_002887278.1
Coordinates 4658719..4658985 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM16_RS22750 (LGM16_22750) 4657006..4658127 + 1122 WP_102086105.1 cupin domain-containing protein -
LGM16_RS22755 (LGM16_22755) 4658175..4658708 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LGM16_RS22760 (LGM16_22760) 4658719..4658985 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LGM16_RS22765 (LGM16_22765) 4659088..4660521 - 1434 WP_019705874.1 Cu(+)/Ag(+) sensor histidine kinase -
LGM16_RS22770 (LGM16_22770) 4660511..4661194 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LGM16_RS22775 (LGM16_22775) 4661366..4662751 + 1386 WP_032409188.1 efflux transporter outer membrane subunit -
LGM16_RS22780 (LGM16_22780) 4662769..4663113 + 345 WP_004192220.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T219525 WP_004178461.1 NZ_CP084830:c4658708-4658175 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T219525 NZ_CP116489:c1043804-1043406 [Haemophilus influenzae]
GTGTTGAAATATATGCTTGATACTAACATTGTGATTTACGTGATTAAGCGTAGGCCATTGGAAATTCTTTCTCGCTTTAA
TCAAAATGCAGGAAAAATGTGCGTGAGTAGCATTACTGTGGCTGAACTCTATTATGGAGCTGAAAAAAGCGAATATCCCG
AAAGAAACATTGCGGTTATTGAAGATTTCTTATCACGCCTCACCATTTTAGATTACCAACCAAAGCATGCAGCTCATTTC
GGCAATATTAAAGCTGAACTCTCTAAACAAGGGAAATTGATTGGCGAAAATGATATTCATATAGCGGCTCATGCTCGTAG
TGAAGGGTTAATTCTGGTTAGTAACAATTTAAGAGAATTTGAGCGAGTTATAGCATTACGTACAGAAAATTGGGTGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT219525 WP_002887278.1 NZ_CP084830:c4658985-4658719 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT219525 NZ_CP116489:c1044037-1043804 [Haemophilus influenzae]
ATGATTGAAGCCTCTGTTTTTATGACTAATCGAAGTCAAGCTGTGCGTTTGCCCGCTGAAGTTCGCTTTTCTGAAGAAAT
CAAAAAATTATCGGTTCGTGTATCGGGCAGTGATCGGATTTTATCGCCTCTCAACCAATCTTGGGATAGTTTTTTCTTGA
ATGATCAAGCAGTTAGCGATGATTTTATGAATGAACGAGAAATTGCATTTCAACCAGAGCGTGAGGCTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References