Detailed information of TA system TA219511

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 352346..352932
• Replicon: chromosome
• Accession: NZ_CP084830
• Organism: Klebsiella pneumoniae strain SB612

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: LGM16_RS01670
• Protein ID: WP_002920800.1
• Coordinates: 352564..352932 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: LGM16_RS01665
• Protein ID: WP_004174006.1
• Coordinates: 352346..352567 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM16_RS01645 (LGM16_01645)	348503..349429	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
LGM16_RS01650 (LGM16_01650)	349426..350703	+	1278	WP_004174005.1	branched chain amino acid ABC transporter permease LivM	-
LGM16_RS01655 (LGM16_01655)	350700..351467	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
LGM16_RS01660 (LGM16_01660)	351469..352182	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
LGM16_RS01665 (LGM16_01665)	352346..352567	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LGM16_RS01670 (LGM16_01670)	352564..352932	+	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LGM16_RS01675 (LGM16_01675)	353205..354521	+	1317	WP_019704662.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
LGM16_RS01680 (LGM16_01680)	354628..355515	+	888	WP_002920792.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
LGM16_RS01685 (LGM16_01685)	355512..356357	+	846	WP_004145129.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
LGM16_RS01690 (LGM16_01690)	356359..357429	+	1071	WP_004150074.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	347332..358166	10834

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T219511 WP_002920800.1 NZ_CP084830:352564-352932 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T219511 NZ_CP116480:3771317-3771604 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTGGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTTACGGCATCGTGGTTATCCTGGTTGCGCATACCATCCGTTTCGAAAGTGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT219511 WP_004174006.1 NZ_CP084830:352346-352567 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT219511 NZ_CP116480:3771591-3771893 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTATACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGCGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAGGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1

Antitoxin

• AlphaFold DB: A0A5E5YJY7