Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5102279..5102904 Replicon chromosome
Accession NZ_CP084829
Organism Klebsiella pneumoniae strain SB615

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LGM17_RS24810 Protein ID WP_002882817.1
Coordinates 5102279..5102662 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LGM17_RS24815 Protein ID WP_004150355.1
Coordinates 5102662..5102904 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM17_RS24795 (5099645) 5099645..5100547 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LGM17_RS24800 (5100544) 5100544..5101179 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LGM17_RS24805 (5101176) 5101176..5102105 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LGM17_RS24810 (5102279) 5102279..5102662 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LGM17_RS24815 (5102662) 5102662..5102904 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LGM17_RS24820 (5103109) 5103109..5104026 + 918 WP_004178029.1 alpha/beta hydrolase -
LGM17_RS24825 (5104040) 5104040..5104981 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LGM17_RS24830 (5105026) 5105026..5105463 - 438 WP_226617377.1 D-aminoacyl-tRNA deacylase -
LGM17_RS24835 (5105460) 5105460..5106320 - 861 WP_004146232.1 virulence factor BrkB family protein -
LGM17_RS24840 (5106314) 5106314..5106913 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T219510 WP_002882817.1 NZ_CP084829:c5102662-5102279 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T219510 NZ_CP116480:3633786-3634136 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT219510 WP_004150355.1 NZ_CP084829:c5102904-5102662 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT219510 NZ_CP116480:3633541-3633792 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References