Detailed information of TA system TA021951

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-AbrB
• Location: 751961..752643
• Replicon: chromosome
• Accession: NC_008818
• Organism: Hyperthermus butylicus DSM 5456

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A2BKV3
• Locus tag: HBUT_RS03995
• Protein ID: WP_011821932.1
• Coordinates: 751961..752377 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A2BKV4
• Locus tag: HBUT_RS04000
• Protein ID: WP_011821933.1
• Coordinates: 752374..752643 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HBUT_RS03980 (Hbut_0757)	747077..748561	+	1485	WP_011821929.1	4Fe-4S binding protein	-
HBUT_RS03985 (Hbut_0758)	748611..749090	+	480	WP_011821930.1	hypothetical protein	-
HBUT_RS03990 (Hbut_0759)	749334..751295	+	1962	WP_011821931.1	hypothetical protein	-
HBUT_RS03995 (Hbut_0760)	751961..752377	-	417	WP_011821932.1	type II toxin-antitoxin system VapC family toxin	Toxin
HBUT_RS04000 (Hbut_0761)	752374..752643	-	270	WP_011821933.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HBUT_RS04005 (Hbut_0762)	753225..754499	-	1275	WP_011821934.1	RsmB/NOP family class I SAM-dependent RNA methyltransferase	-
HBUT_RS04010	754778..755122	+	345	WP_048061458.1	hypothetical protein	-
HBUT_RS04015 (Hbut_0763)	755333..756283	+	951	WP_011821935.1	glycosyltransferase family 2 protein	-
HBUT_RS08905 (Hbut_0764)	756251..757111	-	861	WP_011821936.1	putative CRISPR-associated protein	-
HBUT_RS04025 (Hbut_0765)	757187..757564	-	378	WP_011821937.1	peptidyl-tRNA hydrolase Pth2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-127)

Antitoxin

• SpoVT-AbrB domain (6-46)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15888.59 Da
• Isoelectric Point: 4.9838

>T21951 WP_011821932.1 NC_008818:c752377-751961 [Hyperthermus butylicus DSM 5456]
MKAFLDTPLLVYLNTIAEDERRLVYESFYLDILSRYKLYTDVLVLDELIYVSKRKYGIPYSVTLDFIEAIVLPYVTVIGL
GEDEYREAARLIAKLNLKPSDALHVAAMKMNGIRLVISEDKELDKVPDVKRVWLTSSP

Nucleotide

• Length: 417 bp

>T21951 NC_008818:c752377-751961 [Hyperthermus butylicus DSM 5456]
TTGAAAGCCTTTCTCGACACCCCACTACTAGTATATCTCAACACTATAGCTGAGGACGAGCGTAGACTCGTCTACGAGAG
CTTCTACCTGGACATACTATCCCGGTACAAGCTCTACACGGATGTACTTGTACTTGACGAGTTAATCTATGTTTCCAAGA
GGAAATACGGTATCCCCTATAGCGTAACGCTAGACTTCATAGAGGCTATAGTGCTGCCCTACGTTACAGTAATAGGGCTT
GGCGAGGATGAGTACAGAGAGGCAGCTAGGCTAATAGCCAAGCTCAACCTTAAACCCTCCGATGCTCTCCACGTAGCAGC
AATGAAGATGAATGGTATAAGGCTAGTGATAAGCGAGGATAAGGAGCTGGATAAAGTACCTGACGTGAAAAGGGTCTGGC
TTACAAGTAGCCCATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10005.61 Da
• Isoelectric Point: 8.5402

>AT21951 WP_011821933.1 NC_008818:c752643-752374 [Hyperthermus butylicus DSM 5456]
MTLKLRVGRKGYIILPKAVREAVGIDEGDEVIVEIRDGILLKPAKKRVDVDRIKELLRAHAARLRSIQGRREPAPGEAAA
DYLEEEFES

Nucleotide

• Length: 270 bp

>AT21951 NC_008818:c752643-752374 [Hyperthermus butylicus DSM 5456]
TTGACCCTCAAGCTCAGGGTTGGCAGGAAGGGCTACATTATACTTCCGAAGGCTGTGAGGGAGGCAGTCGGCATCGACGA
GGGTGACGAGGTGATAGTTGAAATAAGGGATGGTATACTTCTAAAGCCCGCTAAGAAGCGGGTTGATGTGGATAGGATCA
AAGAGCTTCTTAGAGCGCATGCGGCAAGGCTGAGAAGCATTCAGGGAAGACGTGAGCCAGCGCCGGGCGAGGCGGCAGCC
GACTATCTCGAGGAGGAGTTTGAAAGTTGA

Structures

Toxin

• AlphaFold DB: A2BKV3

Antitoxin

• AlphaFold DB: A2BKV4