Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4528531..4529341 Replicon chromosome
Accession NZ_CP084829
Organism Klebsiella pneumoniae strain SB615

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LGM17_RS22145 Protein ID WP_004178461.1
Coordinates 4528531..4529064 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LGM17_RS22150 Protein ID WP_002887278.1
Coordinates 4529075..4529341 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM17_RS22140 (4527362) 4527362..4528483 + 1122 WP_004214138.1 cupin domain-containing protein -
LGM17_RS22145 (4528531) 4528531..4529064 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LGM17_RS22150 (4529075) 4529075..4529341 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LGM17_RS22155 (4529444) 4529444..4530877 - 1434 WP_226617344.1 Cu(+)/Ag(+) sensor histidine kinase -
LGM17_RS22160 (4530867) 4530867..4531550 - 684 WP_226617345.1 copper response regulator transcription factor CusR -
LGM17_RS22165 (4531722) 4531722..4533104 + 1383 WP_226617346.1 efflux transporter outer membrane subunit -
LGM17_RS22170 (4533122) 4533122..4533466 + 345 WP_226617520.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T219507 WP_004178461.1 NZ_CP084829:c4529064-4528531 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T219507 NZ_CP116480:c2873951-2873844 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT219507 WP_002887278.1 NZ_CP084829:c4529341-4529075 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT219507 NZ_CP116480:2873999-2874065 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References