Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5223974..5224599 Replicon chromosome
Accession NZ_CP084825
Organism Klebsiella pneumoniae strain SB617

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LGM18_RS25590 Protein ID WP_002882817.1
Coordinates 5223974..5224357 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LGM18_RS25595 Protein ID WP_004150355.1
Coordinates 5224357..5224599 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM18_RS25575 (5221340) 5221340..5222242 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LGM18_RS25580 (5222239) 5222239..5222874 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LGM18_RS25585 (5222871) 5222871..5223800 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LGM18_RS25590 (5223974) 5223974..5224357 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LGM18_RS25595 (5224357) 5224357..5224599 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LGM18_RS25600 (5224804) 5224804..5225721 + 918 WP_012737090.1 alpha/beta hydrolase -
LGM18_RS25605 (5225735) 5225735..5226676 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LGM18_RS25610 (5226721) 5226721..5227158 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LGM18_RS25615 (5227155) 5227155..5228015 - 861 WP_004146232.1 virulence factor BrkB family protein -
LGM18_RS25620 (5228009) 5228009..5228608 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T219494 WP_002882817.1 NZ_CP084825:c5224357-5223974 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T219494 NZ_CP116480:c365904-365797 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT219494 WP_004150355.1 NZ_CP084825:c5224599-5224357 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT219494 NZ_CP116480:365951-366017 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References