Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4650747..4651557 Replicon chromosome
Accession NZ_CP084825
Organism Klebsiella pneumoniae strain SB617

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LGM18_RS22865 Protein ID WP_004178461.1
Coordinates 4650747..4651280 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LGM18_RS22870 Protein ID WP_002887278.1
Coordinates 4651291..4651557 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM18_RS22860 (4649578) 4649578..4650699 + 1122 WP_009309849.1 cupin domain-containing protein -
LGM18_RS22865 (4650747) 4650747..4651280 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LGM18_RS22870 (4651291) 4651291..4651557 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LGM18_RS22875 (4651660) 4651660..4653093 - 1434 WP_023280821.1 Cu(+)/Ag(+) sensor histidine kinase -
LGM18_RS22880 (4653083) 4653083..4653766 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LGM18_RS22885 (4653939) 4653939..4655324 + 1386 WP_117040150.1 efflux transporter outer membrane subunit -
LGM18_RS22890 (4655342) 4655342..4655686 + 345 WP_117040149.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T219490 WP_004178461.1 NZ_CP084825:c4651280-4650747 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T219490 NZ_CP116478:c6267222-6266932 [Pseudomonas syringae pv. actinidiae]
ATGACTTATAGCCTCGAATTTGATGCGCGAGCTCTGAAGGAATGGCACAAATTAGGCGATACCGTGCGTCAGCAGCTCAA
GAAAAAGCTGGCTACGATTCTGGTTGCTCCGCGTGTTGAAGCAAATCGCCTGCATGCGTTGCCCGACTGCTACAAAATCA
AGCTGCGCAGCAGCGGCTATCGGCTGGTTTATCAGGTTATTGACCAAGAGGTTGTGGTCTTCGTGGTTGCTGTGGACAAG
CGTGAGCGAGATGAGGTTTACCGTAAGGCGGCTGATCGGTTGGGTGGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT219490 WP_002887278.1 NZ_CP084825:c4651557-4651291 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT219490 NZ_CP116478:c6267451-6267212 [Pseudomonas syringae pv. actinidiae]
ATGGCGTCTATTAATGTTCGTATCGACGACGATCTCAAGGCTCGTGCTTATCTCGAGCTTGAAAAGCTTGGCGTCACCCC
TTCGGAACTGCTGCGCCAAACCCTGCAGTACGTGGCAGAGCGCGGCCAGTTGCCGTTCAAGACCGTACTGATGACCGAGG
AAGACGAAGCCTTGCTTGCAACAGTGCGCGAGCGCCTTGCGGCTCCGCAACGGGTCAAGGTTTCACTGGATGACTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References