Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4018236..4018855 Replicon chromosome
Accession NZ_CP084825
Organism Klebsiella pneumoniae strain SB617

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LGM18_RS19840 Protein ID WP_002892050.1
Coordinates 4018637..4018855 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LGM18_RS19835 Protein ID WP_002892066.1
Coordinates 4018236..4018610 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM18_RS19825 (4013388) 4013388..4014581 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LGM18_RS19830 (4014604) 4014604..4017750 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
LGM18_RS19835 (4018236) 4018236..4018610 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LGM18_RS19840 (4018637) 4018637..4018855 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LGM18_RS19845 (4019018) 4019018..4019584 + 567 WP_002892030.1 maltose O-acetyltransferase -
LGM18_RS19850 (4019556) 4019556..4019696 - 141 WP_004147370.1 hypothetical protein -
LGM18_RS19855 (4019717) 4019717..4020187 + 471 WP_002892026.1 YlaC family protein -
LGM18_RS19860 (4020162) 4020162..4021613 - 1452 WP_004183206.1 PLP-dependent aminotransferase family protein -
LGM18_RS19865 (4021714) 4021714..4022412 + 699 WP_117040048.1 GNAT family protein -
LGM18_RS19870 (4022409) 4022409..4022549 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LGM18_RS19875 (4022549) 4022549..4022812 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T219488 WP_002892050.1 NZ_CP084825:4018637-4018855 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T219488 NZ_CP116478:5650492-5650911 [Pseudomonas syringae pv. actinidiae]
ATGATCCTGCTCGATACGAACGTAATTTCAGAACCGCAGCGCCGAGAGCCCAATGCCCACGTCCTTGACTGGATCGACGC
ACAAGCGCTGGAAACGCTTTATCTGTCCACCATCACGGTAGCCGAGTTGCGTGCTGGTATTGCGCTGATGCCTGTCGGAA
AACGCCAGGATAGCTTGCGTGAAAACCTGGAAAAGCACCTGCTGCCGATGTTCGCCAATAGGGTGCTGTCGTTCGATATG
ACTTGTACGAAGGCTTATGCCGAGCTGCTGGCCAAGTCCCGCGCAGCCGGGTTGGCGATCGAAACGGCAGATGCCTTCAT
TGCAGCCATCGCCCTCGCCAACGGCTTCACCGTCGCCACCCGTGACACTGGCCCTTTTGAGGCTGCCGGGTTGAACGTCA
TTAACCCCTGGGAAGCGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT219488 WP_002892066.1 NZ_CP084825:4018236-4018610 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT219488 NZ_CP116478:5650238-5650495 [Pseudomonas syringae pv. actinidiae]
GTGGCAAACGTGAACGTAAGAAATCTACCCGATGAAGTGCATCGGGCAATCCGCATCCAAGCTGCCCTGCACGGGCGCAG
CACCGAGGCCGAAATACGCGACATCTTGGAGCGGGCGGCGAGACCCGAGGGACGTGTGAAACTTGGCTCGTTCCTGGCTT
CTATTGCCCGTGAGGTTGGTGGTCTGACCGATAAAGAACACACCTTGTTTGAGAACACGCGCATCACGTCTCCGGCCCGT
GCGGTGAGCTTTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References