Detailed information of TA system TA219477

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 715804..716579
• Replicon: chromosome
• Accession: NZ_CP084825
• Organism: Klebsiella pneumoniae strain SB617

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: LGM18_RS03560
• Protein ID: WP_117046505.1
• Coordinates: 716094..716579 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A7U2ZSW0
• Locus tag: LGM18_RS03555
• Protein ID: WP_025710477.1
• Coordinates: 715804..716097 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM18_RS03535 (711012)	711012..711614	-	603	WP_004174410.1	short chain dehydrogenase	-
LGM18_RS03540 (711712)	711712..712623	+	912	WP_004188550.1	LysR family transcriptional regulator	-
LGM18_RS03545 (712624)	712624..713772	-	1149	WP_114502093.1	PLP-dependent aspartate aminotransferase family protein	-
LGM18_RS03550 (713783)	713783..715159	-	1377	WP_165453336.1	cystathionine beta-synthase	-
LGM18_RS03555 (715804)	715804..716097	+	294	WP_025710477.1	DUF1778 domain-containing protein	Antitoxin
LGM18_RS03560 (716094)	716094..716579	+	486	WP_117046505.1	GNAT family N-acetyltransferase	Toxin
LGM18_RS03565 (716758)	716758..717210	-	453	WP_117040079.1	hypothetical protein	-
LGM18_RS03570 (717325)	717325..717544	-	220	Protein_700	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (41-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17627.66 Da
• Isoelectric Point: 8.5144

>T219477 WP_117046505.1 NZ_CP084825:716094-716579 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRMIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Nucleotide

• Length: 486 bp

>T219477 NZ_CP116477:2924617-2925036 [Pectobacterium sp. A5351]
ATGACAGATATCTTGCTCAACAAAAGCAGCACCATACAAAGATGCCTACGTCGTATTCGTGAGGAATTCGATACTGAGGA
AGGGTTTCGTCAAAACTTCACCAAGCAAGATTCCGTCATACTTAACATACAGCGTGCCTGTGAAGCCGCTATTGATATCG
CTAATTATCTTATCCGTATCAAGCAGCTAGGCATACCACAAAGTAGCCGTGATAGCTTCGCGCTTCTTGCTGCAAACCAG
ATTATTACGGTTGATCTGTCTGACAATTTGCAAAAAATGGTGGGATTAAGAAATATTGCCGTACATGACTATCAGGCGCT
CAATCTGGATATTGTCATCCACGTCATTTCTCATCGTTTAAGCGACTTTGAACATTTTATCAAACAAATAGCCCGGTACA
GCGACAGCCAGCACCTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10990.61 Da
• Isoelectric Point: 8.6141

>AT219477 WP_025710477.1 NZ_CP084825:715804-716097 [Klebsiella pneumoniae]
MPAANSMTMKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPEPN
AALRKTMQTPAPWEQEK

Nucleotide

• Length: 294 bp

>AT219477 NZ_CP116477:2924237-2924620 [Pectobacterium sp. A5351]
ATGTTCGATAAGATCGTTTTGACACTGAAAAAACAGATGCCCGACATCAAAATTATTTATCTCTTTGGCTCCCAGGCTAC
AGGCAATGCCAGAGCGGACAGCGACATTGATATCGCCATCATGGCTACAAGAGCACTGGATCCCGTTAAACGCTGGGAGC
TATCTAATCAATTAGCAAAGGAAGTGGGTCATGACGTCGATCTTATCGATTTATTGCAGGCTTCAACGGTATTAAAAATG
GAGATCGTTCGTAACGGCAAGCTGCTTTATGATGCGGAAACAGCAGCGGGGGAATTTGAAATGACAACACTTTCGATGTA
CCAGCATCTGCAAAAAGAGCGTGCGGATATCATTCGTAGCTTTAATCAGGATCTAAAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A7U2ZSW0