Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 199485..200395 Replicon plasmid pKp_T69_1
Accession NZ_CP084824
Organism Klebsiella pneumoniae strain T69

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag LGM19_RS27000 Protein ID WP_004026354.1
Coordinates 199485..199955 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag LGM19_RS27005 Protein ID WP_004026357.1
Coordinates 199952..200395 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM19_RS26985 (LGM19_26985) 196342..197310 + 969 WP_004225014.1 IS5 family transposase -
LGM19_RS26990 (LGM19_26990) 197968..198228 + 261 WP_004026352.1 hypothetical protein -
LGM19_RS26995 (LGM19_26995) 198842..199030 + 189 WP_223271245.1 hypothetical protein -
LGM19_RS27000 (LGM19_27000) 199485..199955 - 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
LGM19_RS27005 (LGM19_27005) 199952..200395 - 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
LGM19_RS27010 (LGM19_27010) 200496..200999 - 504 Protein_216 DUF4113 domain-containing protein -
LGM19_RS27015 (LGM19_27015) 201097..201318 - 222 WP_004213643.1 DUF2188 domain-containing protein -
LGM19_RS27020 (LGM19_27020) 201499..201618 - 120 Protein_218 Fe3+-citrate ABC transporter substrate-binding protein -
LGM19_RS27025 (LGM19_27025) 201693..203819 - 2127 WP_004213640.1 TonB-dependent Fe(3+) dicitrate receptor FecA -
LGM19_RS27030 (LGM19_27030) 203906..204859 - 954 Protein_220 fec operon regulator FecR -
LGM19_RS27035 (LGM19_27035) 204856..205377 - 522 WP_004213635.1 ferric citrate uptake sigma factor FecI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 1..206626 206626


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T219475 WP_004026354.1 NZ_CP084824:c199955-199485 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T219475 NZ_CP116477:c2628620-2628306 [Pectobacterium sp. A5351]
ATGCAATTCACCGTATACGGGAATACCGGGAAAAGCGTCGTTTACCCGCTGTTGCTCGATGTCACGAGCGATATTATTGG
GCAATTGAATCGTCGGATAGTGATCCCATTGCTCCCTATTGAAAGGTATCCGGCAGGCCGCCGACCGGAGCGCCTTGTCC
CCGTCGTCAGGCTGACGGACGGCAAAGAATACGCCGTAATGACCCACGAGTTGGCAAGTATCCCTGTCCAGGCACTGGGT
GCGGTGTTTTGTGATGCGTCGCAGTACCGTACTCAGATAAAGGCGGCAATAGACTTCCTCATCGACGGGTTCTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT219475 WP_004026357.1 NZ_CP084824:c200395-199952 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT219475 NZ_CP116477:c2628859-2628623 [Pectobacterium sp. A5351]
ATGACCGCGAAACAACGCAACACGCAGAGTGTGACCATGACAGTAGAGCGTGCCTTACTGGTAAGAGCGCGTGAGGCGGG
TATTAATCTGAGTGCTACCCTGACAACCGCCCTGGATGCAGAGCTTCGCTCTCATGAAGCGAAAAAATGGCAGGAAGAGA
ACAGGGAGGCTCTCGCAGCACTCAATCGTTTTCATGATGAGCAGGGTTGTTTCAGCGATGAATACAGGACGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References