Detailed information of TA system TA219472

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 7987..8897
• Replicon: plasmid pKp_T69_1
• Accession: NZ_CP084824
• Organism: Klebsiella pneumoniae strain T69

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: LGM19_RS25985
• Protein ID: WP_004026354.1
• Coordinates: 7987..8457 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: LGM19_RS25990
• Protein ID: WP_004026357.1
• Coordinates: 8454..8897 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM19_RS25960 (LGM19_25960)	3262..3911	-	650	Protein_3	transposase	-
LGM19_RS25965 (LGM19_25965)	3980..4507	-	528	WP_267461751.1	RES domain-containing protein	-
LGM19_RS27090	4467..4859	-	393	WP_267461752.1	RES domain-containing protein	-
LGM19_RS25970 (LGM19_25970)	4839..5812	+	974	Protein_6	IS5 family transposase	-
LGM19_RS25975 (LGM19_25975)	6470..6730	+	261	WP_004026352.1	hypothetical protein	-
LGM19_RS25980 (LGM19_25980)	7344..7532	+	189	WP_223271245.1	hypothetical protein	-
LGM19_RS25985 (LGM19_25985)	7987..8457	-	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
LGM19_RS25990 (LGM19_25990)	8454..8897	-	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
LGM19_RS25995 (LGM19_25995)	8998..9501	-	504	Protein_11	DUF4113 domain-containing protein	-
LGM19_RS26000 (LGM19_26000)	9599..9820	-	222	WP_004213643.1	DUF2188 domain-containing protein	-
LGM19_RS26005 (LGM19_26005)	10001..10120	-	120	Protein_13	Fe3+-citrate ABC transporter substrate-binding protein	-
LGM19_RS26010 (LGM19_26010)	10195..12321	-	2127	WP_004213640.1	TonB-dependent Fe(3+) dicitrate receptor FecA	-
LGM19_RS26015 (LGM19_26015)	12408..13361	-	954	Protein_15	fec operon regulator FecR	-
LGM19_RS26020 (LGM19_26020)	13358..13879	-	522	WP_004213635.1	ferric citrate uptake sigma factor FecI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..206626	206626

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T219472 WP_004026354.1 NZ_CP084824:c8457-7987 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T219472 NZ_CP116477:c889726-889421 [Pectobacterium sp. A5351]
ATGCAATTCATTGTTTATCAATACAAACGAGCCAGTCACTACAAAATGTTTGTTGATGTACAAAGTGATATTGTCGAGAC
ACCGAAGCGGCGCATGGTCATCCCGTTTATTGAATCACATCACCTGTCTGAGAAAGTGAATAAAACGCTGTTCCCGCTGA
TTCGCATCAATGACGAAGATTATCGGCTGATGACGACTGAACTATCGAGCGTTCCCGTTGAAGTTATGGGTGAAGTGATT
GCGGATCTCAGCGACTGCGCGAATGAGATCAAGGATGCTATCAATCTTATGTTTTGGGGCATTTAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT219472 WP_004026357.1 NZ_CP084824:c8897-8454 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT219472 NZ_CP116477:c889947-889729 [Pectobacterium sp. A5351]
ATGAAACACCGCATCAGCGTTACCGTGGACAAAGACAATTATCAGGTTCTGAGTACTGCCGGAGTCAATATTTCCGGGCT
GGTGAATGATGCCATTGGCAAAGAAGCTCGCCGCATCAAGGTAGAGGAGTGGAAGAAGGAAAGCCGCGAAGGGATGGAAG
AAGTCGCCCGATTTATCGCGCAGCATGGCTCCTTTGCAGATGAAAACAGGAACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A6P1V3Q9