Detailed information of TA system TA219462

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 3421497..3423085
• Replicon: chromosome
• Accession: NZ_CP084823
• Organism: Klebsiella pneumoniae strain T69

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: W8UI36
• Locus tag: LGM19_RS16565
• Protein ID: WP_002904792.1
• Coordinates: 3421763..3423085 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LGM19_RS16560
• Protein ID: WP_004176198.1
• Coordinates: 3421497..3421763 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM19_RS16535 (3416526)	3416526..3417449	+	924	WP_032458772.1	LysR family hpxDE operon transcriptional regulator HpxR	-
LGM19_RS16540 (3417430)	3417430..3418584	-	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
LGM19_RS16545 (3418930)	3418930..3420309	+	1380	WP_014599164.1	nucleobase:cation symporter-2 family protein	-
LGM19_RS16550 (3420287)	3420287..3420787	+	501	WP_004224756.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
LGM19_RS16555 (3420784)	3420784..3421110	+	327	WP_004224757.1	hydroxyisourate hydrolase	-
LGM19_RS16560 (3421497)	3421497..3421763	+	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LGM19_RS16565 (3421763)	3421763..3423085	+	1323	WP_002904792.1	type II toxin-antitoxin system HipA family toxin	Toxin
LGM19_RS16570 (3423102)	3423102..3424394	-	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
LGM19_RS16575 (3424536)	3424536..3425540	-	1005	WP_004224758.1	fimbrial protein	-
LGM19_RS16580 (3425528)	3425528..3428029	-	2502	WP_032458773.1	fimbria/pilus outer membrane usher protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49171.46 Da
• Isoelectric Point: 7.5357

>T219462 WP_002904792.1 NZ_CP084823:3421763-3423085 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T219462 NZ_CP116477:c32615-32229 [Pectobacterium sp. A5351]
ATGGAGGTTTACAGATTGGCATGGGAAATAGTCACACGGGCGCTATTCGATACGTGGTTTGATGAGCAAACCGAAACGGT
ACAGGAAGAGGTTTTGGCACACCTCGGTATACTCGAAGAAGATGGCCCACAGCTAGGGCGACCACAGGTTGATCAGATTA
AAGGCTCACAATACAAAAATATGAAGGAGCTTCGAGTTCAGGTTGGCGACCACCCATTCCGAACATTCTTCGCGTTTGAT
CGGACACGTAAAGCGATTGTTTTATGTGCGGGAGATAAGAAAGGCGAAAATGAAAAATTGTTCTACGAACGTATGATTAA
GATCGCAGACGCTGAGTTTACATCGCATCTGATATCACCGGAGGTAAAAGAAGATGGCGACATTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT219462 WP_004176198.1 NZ_CP084823:3421497-3421763 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT219462 NZ_CP116477:c32242-31943 [Pectobacterium sp. A5351]
ATGGCGACATTTAAAGAAATGATGGCTAAACGTTCACCGGAGAGCCGCGCCCGCATTGAAGCGCGCACGGCTGAGATCCG
TCAGGAAATCACTTTAGCGAAACTACGCGAAGAGCTGAACATGTCGCAGACTCAGCTTGCATTAGCTCTGGGCGTCAAGC
AGCCATCTATTGTTAAGATGGAGAAGGTCGAGAATGACCCAAAACTGTCAACGCTGAAACGCTATGTCACTGCCCTAGGC
GGCAAGCTTACGCTAGATGTGACCTTGCCTGATGGTAAGCGTATCGCGCTTACTTTGTAA

Structures

Toxin

• AlphaFold DB: A0A0E1CEC8