Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2066652..2067271 Replicon chromosome
Accession NZ_CP084823
Organism Klebsiella pneumoniae strain T69

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LGM19_RS09925 Protein ID WP_002892050.1
Coordinates 2066652..2066870 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LGM19_RS09930 Protein ID WP_002892066.1
Coordinates 2066897..2067271 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM19_RS09890 (2062699) 2062699..2062962 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
LGM19_RS09895 (2062962) 2062962..2063102 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LGM19_RS09900 (2063099) 2063099..2063797 - 699 WP_002892021.1 GNAT family protein -
LGM19_RS09905 (2063898) 2063898..2065349 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
LGM19_RS09910 (2065324) 2065324..2065794 - 471 WP_002892026.1 YlaC family protein -
LGM19_RS09915 (2065815) 2065815..2065955 + 141 WP_004147370.1 hypothetical protein -
LGM19_RS09920 (2065927) 2065927..2066493 - 567 WP_002892030.1 maltose O-acetyltransferase -
LGM19_RS09925 (2066652) 2066652..2066870 - 219 WP_002892050.1 HHA domain-containing protein Toxin
LGM19_RS09930 (2066897) 2066897..2067271 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LGM19_RS09935 (2067757) 2067757..2070903 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
LGM19_RS09940 (2070926) 2070926..2072119 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T219461 WP_002892050.1 NZ_CP084823:c2066870-2066652 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T219461 NZ_CP116458:c1862791-1862369 [Acidithiobacillus ferriphilus]
GTGAGATACCTCCTGGATACCTGCGTTCTGTCAGAGTTGGTCAAAAAGCATCCTGATCCTCGGGTCGTAACATGGGTAGA
CGCACGGGAGGCTGTGGGGTTTCTTAGTGTGCTCACACTGGGAGAGCTCCAAAAAGGCGTTGCCAAACTTGTTGACGGCG
ATAAGCGATCAATGCTGCAGGATTGGATAGACGTTGATCTGCGAGGTCGTTTTGTCGGACGTATTCTTCCTGTTAACGAG
CATATCGCCAGTGCATGGGGAGGTATAACAGGTGCCGCAGAACAACGCGGAGAGAAGTTGCCAGCGATAGATAGCTTGCT
GGCAGCCACGGCGCTGGTTCACCATCTCACAGTGGCCACGCGGAATATTGTTGATTTACAGCGATGCCAAGTTCCGGTGG
TCGATCCCTGGGATCAAACATAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT219461 WP_002892066.1 NZ_CP084823:c2067271-2066897 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT219461 NZ_CP116458:c1863042-1862788 [Acidithiobacillus ferriphilus]
ATGAATACTCAGTGGCCTTTACAGGAGGCGCGCAATCATTTGAGCGATGTCGTGAAGCGTGCCCGCAATGATGGTCCGCA
AACCATTACCTTACATGGTGAGCCCGCGGTTGTAGTGCTCTCCGTAGAACAGTTTCGGACGTTGACACGGCCGAACCAGA
CGTTATCCGAATTCCTCGCGAACTCTCCACTGCGAGGGCTCGATCTGGAAGTCGAACGAGATCATCACGATGTGGGGCGC
GACGTCAGCCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References