Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 1462482..1463292 Replicon chromosome
Accession NZ_CP084823
Organism Klebsiella pneumoniae strain T69

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LGM19_RS07020 Protein ID WP_004178461.1
Coordinates 1462759..1463292 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A483I2M4
Locus tag LGM19_RS07015 Protein ID WP_004222378.1
Coordinates 1462482..1462748 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM19_RS06995 (1458354) 1458354..1458698 - 345 WP_004222368.1 cation efflux system protein CusF -
LGM19_RS07000 (1458716) 1458716..1460101 - 1386 WP_004222371.1 efflux transporter outer membrane subunit -
LGM19_RS07005 (1460273) 1460273..1460956 + 684 WP_002887273.1 copper response regulator transcription factor CusR -
LGM19_RS07010 (1460946) 1460946..1462379 + 1434 WP_004222374.1 Cu(+)/Ag(+) sensor histidine kinase -
LGM19_RS07015 (1462482) 1462482..1462748 + 267 WP_004222378.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LGM19_RS07020 (1462759) 1462759..1463292 + 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LGM19_RS07025 (1463340) 1463340..1464461 - 1122 WP_002887282.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T219460 WP_004178461.1 NZ_CP084823:1462759-1463292 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T219460 NZ_CP116458:c1846311-1846129 [Acidithiobacillus ferriphilus]
ATGGAAAAAGGTATTCTTCACTGCAAGTTGGCCACCGTAAAGGCATTGACCGAAGCCGGTAAGGTCAACACTACGGCCAG
CGCCTTCAAGGGCGCCCGTGAATTGGGGATCAATGATCTTGCCGGGATGTGTACCATGCCAAGACAGATAACAGTGCTAA
TTGTTTCTTTCAAGGAGTTGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9969.44 Da        Isoelectric Point: 10.1757

>AT219460 WP_004222378.1 NZ_CP084823:1462482-1462748 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLSEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT219460 NZ_CP116458:c1846126-1845719 [Acidithiobacillus ferriphilus]
ATGAAATGCCCATGCTGTGGCGTGGCTGAATTGATCCATGATACCCGCGATATGCCCTACATATACAAGGGCGAAACCAC
CACTATTCCAGCGGTGACTGGCGACTTCTGCCCGGCGTGTGGCGAGGTTGTCCTGAACCGCGAATATGGCGGCCGTTACA
GTGAGTTGATTGGGCAATTCCAGCGTCAGGTGAACTCGGCCTATGTTGATCCTCACTACATCGCTCTGGTGCGCAAGAAG
CTTGATCTGGATCAACGCCAAGCCGCTGCAATCTTCGGCGGCGGAGCCAATGCCTTCTCGCGTTACGAAAACGGCAAGAC
CAAGCCTTCTTTGGCGCTGGTGAAGCTGCTCAAGGTGCTTGAACGGCATCCCGATTTGCTTAACGAGGTTAAATCCGGGC
CTGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
AlphaFold DB A0A483I2M4

References