Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 847783..848408 Replicon chromosome
Accession NZ_CP084823
Organism Klebsiella pneumoniae strain T69

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LGM19_RS04150 Protein ID WP_002882817.1
Coordinates 848025..848408 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LGM19_RS04145 Protein ID WP_004150355.1
Coordinates 847783..848025 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM19_RS04120 (843774) 843774..844373 + 600 WP_004901669.1 glucose-1-phosphatase -
LGM19_RS04125 (844367) 844367..845227 + 861 WP_002882807.1 virulence factor BrkB family protein -
LGM19_RS04130 (845224) 845224..845661 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LGM19_RS04135 (845706) 845706..846647 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LGM19_RS04140 (846661) 846661..847578 - 918 WP_004901674.1 alpha/beta hydrolase -
LGM19_RS04145 (847783) 847783..848025 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LGM19_RS04150 (848025) 848025..848408 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LGM19_RS04155 (848582) 848582..849511 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LGM19_RS04160 (849508) 849508..850143 - 636 WP_004901683.1 formate dehydrogenase cytochrome b556 subunit -
LGM19_RS04165 (850140) 850140..851042 - 903 WP_004901686.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T219458 WP_002882817.1 NZ_CP084823:848025-848408 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T219458 NZ_CP116458:1326507-1326938 [Acidithiobacillus ferriphilus]
ATGATCTTCTTGCTCGATGTAAACGTGCTGATTGCGCTCATCGACCCCATGCATGTTCAGCATGATATGGCCCATGAATG
GTTTGCAGCCCATGGGCAACCCGCATGGGCTACCTGTCCCTTAACAGAGAATGGTGTACTGCGCATTGTGAGCCATCCGC
GCTATCCAAATTCGCCAGGCACGCCAGCGGCAGTGGCACCCCTGATTCAGGGATTGCGCGCGTCACCTGGCCATGTTTTT
TGGCCAGATGACATCAGCCTGCTGGATGTCGATCATATCGACGCATCGCGTCTGCTAACTTCTGGTCAGATTACCAATAG
CTACCTCTTGGCGCTGGCCTGCGCCCATGAGGGCCAACTGGCAACCTTCGATCATCGCTTAGTGACCAATGCCGTGCGCA
ACAGTTTGCAATGCATAAACAAAATTAAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT219458 WP_004150355.1 NZ_CP084823:847783-848025 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT219458 NZ_CP116458:1326274-1326507 [Acidithiobacillus ferriphilus]
ATGCGCACCACACTGGCGATTGATGATGACGTACTGGCGGCGGCGAAAGGGCTAGCGGATCGACAGCATAAGCCTGTAGG
TGCCGTTGTTTCAGCACTTTTGCGGCAGGCTCTGCAATCACAAAACACCTCTGGTGGTCATTCCCGTAATGGGATTCCCT
TGCTGTCGGTGCGCCCGGATGCACTCCCGGCGACTCTGGAAATAGTGAATCAGTTGCGCGACGAATTGCCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References