Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 52817..53460 Replicon plasmid pKqq_18A069_2
Accession NZ_CP084820
Organism Klebsiella quasipneumoniae subsp. quasipneumoniae strain 18A069

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag LGM21_RS26805 Protein ID WP_001044770.1
Coordinates 52817..53233 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag LGM21_RS26810 Protein ID WP_001261282.1
Coordinates 53230..53460 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM21_RS26795 (LGM21_26795) 50174..51196 - 1023 WP_000361404.1 helicase UvrD -
LGM21_RS26800 (LGM21_26800) 51181..52743 - 1563 WP_004206609.1 AAA family ATPase -
LGM21_RS26805 (LGM21_26805) 52817..53233 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
LGM21_RS26810 (LGM21_26810) 53230..53460 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LGM21_RS26815 (LGM21_26815) 53417..53878 + 462 WP_072202616.1 hypothetical protein -
LGM21_RS26820 (LGM21_26820) 54022..54372 + 351 WP_000493378.1 hypothetical protein -
LGM21_RS26825 (LGM21_26825) 54423..55166 + 744 WP_000129823.1 hypothetical protein -
LGM21_RS26830 (LGM21_26830) 55163..55939 + 777 WP_000015958.1 site-specific integrase -
LGM21_RS26835 (LGM21_26835) 55997..56254 - 258 WP_000764642.1 hypothetical protein -
LGM21_RS26840 (LGM21_26840) 56383..56487 - 105 WP_032409716.1 hypothetical protein -
LGM21_RS26845 (LGM21_26845) 57022..57888 + 867 WP_004118283.1 replication initiation protein -
LGM21_RS26850 (LGM21_26850) 58245..58442 - 198 WP_044296379.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sul1 / qacE / ant(3'')-Ia - 1..75062 75062


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T219455 WP_001044770.1 NZ_CP084820:c53233-52817 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T219455 NZ_CP116458:302209-302487 [Acidithiobacillus ferriphilus]
ATGCGGACGACTAAACAAACCAGCCAGTTCAAGCGTGATCTGAAGAGAGAGTCGAAAGGGCCTTACCGAAAATCGCTCCA
GGAAGGTTTTGTAGCATTGATAGAGGCGTTGGCGGCGGATCTGGTACTGGACCAAAAATATCTCGATCACCCGTTGTCTG
GTGACTGGATAGACCACAGGGATTGCCACTTCAAGCCGGATTTGGTGTTGATCTATCGAAAGACCGAAGGGGTTTTGCAA
CTGGTCCGGTTGGGGTCGCACAGTGAGCTGGGGCTATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT219455 WP_001261282.1 NZ_CP084820:c53460-53230 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT219455 NZ_CP116458:301962-302222 [Acidithiobacillus ferriphilus]
ATGTCTGCAAACGCCGTAGTACGTGCCAGGATCGACGAGCACATTAAGGAAGAAGCTACTGTTGTGCTGGCAGCGATGGG
GTTGACGGTATCGGATGCGTTCCGATTGATGCTGACCCGTGTGGCGCGTGAAAAGCTATTACCCTTTGACCCATTGGTGC
CCAATGAGAAAACCATAGAGGCCATGAAGGAAGCCCGTCAGGGGCATTTGGAGACGGTCACGCTCCAGGATTTGCAAGCG
GTTCTTGATGCGGACGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References