Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 28055..28580 Replicon plasmid pKqq_18A069_1
Accession NZ_CP084819
Organism Klebsiella quasipneumoniae subsp. quasipneumoniae strain 18A069

Toxin (Protein)


Gene name ccdB Uniprot ID A0A6S4YF27
Locus tag LGM21_RS26055 Protein ID WP_025714517.1
Coordinates 28275..28580 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag LGM21_RS26050 Protein ID WP_001568025.1
Coordinates 28055..28273 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM21_RS26040 (LGM21_26040) 25737..26765 - 1029 WP_001568023.1 Abi family protein -
LGM21_RS26045 (LGM21_26045) 26906..27475 - 570 WP_016162066.1 hypothetical protein -
LGM21_RS26050 (LGM21_26050) 28055..28273 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
LGM21_RS26055 (LGM21_26055) 28275..28580 + 306 WP_025714517.1 type II toxin-antitoxin system toxin CcdB Toxin
LGM21_RS26060 (LGM21_26060) 28749..29144 + 396 WP_017899885.1 hypothetical protein -
LGM21_RS26065 (LGM21_26065) 29171..29485 + 315 WP_053389906.1 hypothetical protein -
LGM21_RS26070 (LGM21_26070) 29496..30512 + 1017 WP_017899884.1 hypothetical protein -
LGM21_RS26075 (LGM21_26075) 30710..31504 + 795 WP_004197635.1 site-specific integrase -
LGM21_RS26080 (LGM21_26080) 31944..32246 - 303 WP_071571079.1 hypothetical protein -
LGM21_RS26085 (LGM21_26085) 32243..32869 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..108548 108548


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11562.25 Da        Isoelectric Point: 5.6906

>T219453 WP_025714517.1 NZ_CP084819:28275-28580 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSCELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T219453 NZ_CP116458:50469-50723 [Acidithiobacillus ferriphilus]
GTGAGGCTGATCTTCTCTGACGATGCATGGGAAGACTACCTATATTGGCAGAAGCAAGACAAGCGCATGGTCGAGCGAAT
CAATAAGCTGATACGTGAGACACAGCGTGAACCCTTCGCTGGAGTTGGCAAACCCGAAGCACTGAAGCATGCTCTTTCAG
GATTTTGGTCACGCCGTATTACAGATGAGCATCGCATGGTGTATCGAATAGAAGGTGATGCATTGATGATTGCTCAGTTG
CGTTTCCACTACTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT219453 WP_001568025.1 NZ_CP084819:28055-28273 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT219453 NZ_CP116458:50221-50472 [Acidithiobacillus ferriphilus]
ATGGATGCGATTACCTATACCACCGTTCGTGCAAACCTCGCCAGCGCCATGGACCGCGTTTGCGACGACCACGAACCCTT
GATCATTACCCGCAATGGCGAACAGTCCGTCGTGATGCTCTCGCTCGAAGACTACAAGGCGCTGGAGGAAACCGCCTACC
TCTTGCGTACCCCCGCCAACGCCAAGCGCCTTCTCGCTGCCGTCGCACAACTGAACGCCGGTAAAGGTGTTGAGCGGAAG
CTGGCGAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6S4YF27

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References