Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5803..6328 Replicon plasmid pKqq_SB1124_4
Accession NZ_CP084810
Organism Klebsiella quasipneumoniae subsp. quasipneumoniae strain SB1124

Toxin (Protein)


Gene name ccdB Uniprot ID W8UQV6
Locus tag LGM22_RS28055 Protein ID WP_001568026.1
Coordinates 5803..6108 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag LGM22_RS28060 Protein ID WP_001568025.1
Coordinates 6110..6328 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM22_RS28025 (LGM22_28025) 1519..2145 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
LGM22_RS28030 (LGM22_28030) 2142..2444 + 303 WP_004197636.1 hypothetical protein -
LGM22_RS28035 (LGM22_28035) 2884..3678 - 795 WP_004197635.1 site-specific integrase -
LGM22_RS28040 (LGM22_28040) 3876..4892 - 1017 WP_020315256.1 hypothetical protein -
LGM22_RS28045 (LGM22_28045) 4889..5212 - 324 WP_022644730.1 hypothetical protein -
LGM22_RS28050 (LGM22_28050) 5239..5634 - 396 WP_017899885.1 hypothetical protein -
LGM22_RS28055 (LGM22_28055) 5803..6108 - 306 WP_001568026.1 type II toxin-antitoxin system toxin CcdB Toxin
LGM22_RS28060 (LGM22_28060) 6110..6328 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
LGM22_RS28065 (LGM22_28065) 6868..7821 + 954 WP_168380100.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
LGM22_RS28070 (LGM22_28070) 7891..8471 + 581 Protein_10 IS1 family transposase -
LGM22_RS28075 (LGM22_28075) 8554..8958 + 405 WP_000839182.1 transposase -
LGM22_RS28080 (LGM22_28080) 8955..9302 + 348 WP_000612626.1 IS66 family insertion sequence element accessory protein TnpB -
LGM22_RS28085 (LGM22_28085) 9351..10889 + 1539 WP_023316587.1 IS66-like element ISKpn24 family transposase -
LGM22_RS28090 (LGM22_28090) 10928..11050 + 123 Protein_14 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..84967 84967


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11645.32 Da        Isoelectric Point: 5.6919

>T219439 WP_001568026.1 NZ_CP084810:c6108-5803 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASAWLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T219439 NZ_CP116411:2669641-2669748 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT219439 WP_001568025.1 NZ_CP084810:c6328-6110 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT219439 NZ_CP116411:c2669585-2669529 [Escherichia coli]
TCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EZN1

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References