Detailed information of TA system TA219437

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 20890..21533
• Replicon: plasmid pKqq_SB1124_1
• Accession: NZ_CP084807
• Organism: Klebsiella quasipneumoniae subsp. quasipneumoniae strain SB1124

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: LGM22_RS25170
• Protein ID: WP_136069913.1
• Coordinates: 21117..21533 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: LGM22_RS25165
• Protein ID: WP_001261282.1
• Coordinates: 20890..21120 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM22_RS25130 (LGM22_25130)	17009..17644	+	636	WP_009309912.1	type II CAAX endopeptidase family protein	-
LGM22_RS25135 (LGM22_25135)	17752..18096	-	345	WP_009309911.1	hypothetical protein	-
LGM22_RS25140 (LGM22_25140)	18419..18949	+	531	WP_223174905.1	hypothetical protein	-
LGM22_RS25145 (LGM22_25145)	18918..19355	+	438	WP_009309910.1	hypothetical protein	-
LGM22_RS25150 (LGM22_25150)	19546..19986	-	441	WP_047057603.1	hypothetical protein	-
LGM22_RS25155 (LGM22_25155)	20005..20628	-	624	WP_223345396.1	hypothetical protein	-
LGM22_RS25160 (LGM22_25160)	20697..20933	-	237	Protein_24	hypothetical protein	-
LGM22_RS25165 (LGM22_25165)	20890..21120	+	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LGM22_RS25170 (LGM22_25170)	21117..21533	+	417	WP_136069913.1	type II toxin-antitoxin system VapC family toxin	Toxin
LGM22_RS25175 (LGM22_25175)	21607..23169	+	1563	WP_009309907.1	AAA family ATPase	-
LGM22_RS25180 (LGM22_25180)	23154..24176	+	1023	WP_000361404.1	helicase UvrD	-
LGM22_RS25185 (LGM22_25185)	24720..25628	+	909	WP_032425603.1	HNH endonuclease	-
LGM22_RS25190 (LGM22_25190)	25814..26164	-	351	WP_004187110.1	DUF305 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..283582	283582

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15124.55 Da
• Isoelectric Point: 7.1084

>T219437 WP_136069913.1 NZ_CP084807:21117-21533 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRMEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T219437 NZ_CP116411:2117159-2117262 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT219437 WP_001261282.1 NZ_CP084807:20890-21120 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT219437 NZ_CP116411:c2117300-2117029 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3