Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 11410..12053 | Replicon | plasmid pKqq_SB1124_1 |
| Accession | NZ_CP084807 | ||
| Organism | Klebsiella quasipneumoniae subsp. quasipneumoniae strain SB1124 | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | A0A3Q8D6L7 |
| Locus tag | LGM22_RS25110 | Protein ID | WP_020804312.1 |
| Coordinates | 11637..12053 (+) | Length | 139 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | Q84A07 |
| Locus tag | LGM22_RS25105 | Protein ID | WP_001261276.1 |
| Coordinates | 11410..11640 (+) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LGM22_RS25065 (LGM22_25065) | 6947..7060 | + | 114 | WP_014343462.1 | hypothetical protein | - |
| LGM22_RS25070 (LGM22_25070) | 7189..7446 | + | 258 | WP_009310077.1 | hypothetical protein | - |
| LGM22_RS25075 (LGM22_25075) | 7504..8283 | - | 780 | WP_023287113.1 | site-specific integrase | - |
| LGM22_RS25080 (LGM22_25080) | 8481..9497 | - | 1017 | WP_136069911.1 | hypothetical protein | - |
| LGM22_RS25085 (LGM22_25085) | 9531..9866 | - | 336 | WP_009309920.1 | hypothetical protein | - |
| LGM22_RS25090 (LGM22_25090) | 9916..10056 | - | 141 | WP_162898808.1 | hypothetical protein | - |
| LGM22_RS25095 (LGM22_25095) | 10285..10590 | - | 306 | WP_009309918.1 | type II toxin-antitoxin system toxin CcdB | - |
| LGM22_RS25100 (LGM22_25100) | 10592..10810 | - | 219 | WP_006788213.1 | type II toxin-antitoxin system antitoxin CcdA | - |
| LGM22_RS25105 (LGM22_25105) | 11410..11640 | + | 231 | WP_001261276.1 | type II toxin-antitoxin system VapB family antitoxin | Antitoxin |
| LGM22_RS25110 (LGM22_25110) | 11637..12053 | + | 417 | WP_020804312.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| LGM22_RS25115 (LGM22_25115) | 12094..12972 | - | 879 | WP_136069912.1 | restriction endonuclease | - |
| LGM22_RS25120 (LGM22_25120) | 13634..14911 | + | 1278 | WP_009309915.1 | HlyD family secretion protein | - |
| LGM22_RS25125 (LGM22_25125) | 14901..17009 | + | 2109 | WP_032427226.1 | peptidase domain-containing ABC transporter | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | - | - | 1..283582 | 283582 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 139 a.a. Molecular weight: 15154.59 Da Isoelectric Point: 7.8637
>T219436 WP_020804312.1 NZ_CP084807:11637-12053 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MKKTWMLDTNICSFIMREQPESVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK
MKKTWMLDTNICSFIMREQPESVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK
Download Length: 417 bp
>T219436 NZ_CP116411:c1202353-1202033 [Escherichia coli]
ATGAAAACTCTACCTGCTACAATTTCGCGGGCGGCGAAGCCCTGTCTGTCGCCTGTTGCAGTCTGGCAAATGCTACTTAC
ACGCCTGCTGGAAAAACACTATGGTCTGACCCTGAACGATACGCCGTTTTGTGATGAAACCGTTATACAGGAACATATCG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAAAGAAATATGAGTTAGTTCGTATCGATCGTAAGGGGTTT
AGCTGGCAAAAACAGTCGCCTTTTATTACTGCTGTAGACATTCTAAAAGCCAGACGGGCTATGAGGGAAATGAGAACTTA
A
ATGAAAACTCTACCTGCTACAATTTCGCGGGCGGCGAAGCCCTGTCTGTCGCCTGTTGCAGTCTGGCAAATGCTACTTAC
ACGCCTGCTGGAAAAACACTATGGTCTGACCCTGAACGATACGCCGTTTTGTGATGAAACCGTTATACAGGAACATATCG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAAAGAAATATGAGTTAGTTCGTATCGATCGTAAGGGGTTT
AGCTGGCAAAAACAGTCGCCTTTTATTACTGCTGTAGACATTCTAAAAGCCAGACGGGCTATGAGGGAAATGAGAACTTA
A
Antitoxin
Download Length: 77 a.a. Molecular weight: 8721.74 Da Isoelectric Point: 4.3533
>AT219436 WP_001261276.1 NZ_CP084807:11410-11640 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP
Download Length: 231 bp
>AT219436 NZ_CP116411:c1202691-1202374 [Escherichia coli]
ATGAGCAACATCACATGGGGCCTGCAGCGGGATATTACGCCACGCCTGGGAGCCCGTCTGGTGCAGGAGGGGAACAGGTT
GCACTATCTGGCTGACCGGGCAAATATTACCGATAAGTTTAGTGATGCCGAATGCCTGAAGTTGGATGTCGCATTTCCGC
ATTTTATCCGTCAGATGGAATCGATGCTGACCACTAGTGAAATGAATCCTCGCCATGCCCACTGTGTCACCCTGTACCAC
AACGGTTTCACCTGCGAAGCCGATACCCTTGGTAGTTGCGGCTACGTATATATCGCCATTTATCCCACTCAACGCTAA
ATGAGCAACATCACATGGGGCCTGCAGCGGGATATTACGCCACGCCTGGGAGCCCGTCTGGTGCAGGAGGGGAACAGGTT
GCACTATCTGGCTGACCGGGCAAATATTACCGATAAGTTTAGTGATGCCGAATGCCTGAAGTTGGATGTCGCATTTCCGC
ATTTTATCCGTCAGATGGAATCGATGCTGACCACTAGTGAAATGAATCCTCGCCATGCCCACTGTGTCACCCTGTACCAC
AACGGTTTCACCTGCGAAGCCGATACCCTTGGTAGTTGCGGCTACGTATATATCGCCATTTATCCCACTCAACGCTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3Q8D6L7 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A387K023 |