Detailed information of TA system TA219435

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 10285..10810
• Replicon: plasmid pKqq_SB1124_1
• Accession: NZ_CP084807
• Organism: Klebsiella quasipneumoniae subsp. quasipneumoniae strain SB1124

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A7H9GHC5
• Locus tag: LGM22_RS25095
• Protein ID: WP_009309918.1
• Coordinates: 10285..10590 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A1D8K3R4
• Locus tag: LGM22_RS25100
• Protein ID: WP_006788213.1
• Coordinates: 10592..10810 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM22_RS25060 (LGM22_25060)	5447..6415	+	969	WP_000654811.1	IS5 family transposase	-
LGM22_RS25065 (LGM22_25065)	6947..7060	+	114	WP_014343462.1	hypothetical protein	-
LGM22_RS25070 (LGM22_25070)	7189..7446	+	258	WP_009310077.1	hypothetical protein	-
LGM22_RS25075 (LGM22_25075)	7504..8283	-	780	WP_023287113.1	site-specific integrase	-
LGM22_RS25080 (LGM22_25080)	8481..9497	-	1017	WP_136069911.1	hypothetical protein	-
LGM22_RS25085 (LGM22_25085)	9531..9866	-	336	WP_009309920.1	hypothetical protein	-
LGM22_RS25090 (LGM22_25090)	9916..10056	-	141	WP_162898808.1	hypothetical protein	-
LGM22_RS25095 (LGM22_25095)	10285..10590	-	306	WP_009309918.1	type II toxin-antitoxin system toxin CcdB	Toxin
LGM22_RS25100 (LGM22_25100)	10592..10810	-	219	WP_006788213.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
LGM22_RS25105 (LGM22_25105)	11410..11640	+	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	-
LGM22_RS25110 (LGM22_25110)	11637..12053	+	417	WP_020804312.1	type II toxin-antitoxin system VapC family toxin	-
LGM22_RS25115 (LGM22_25115)	12094..12972	-	879	WP_136069912.1	restriction endonuclease	-
LGM22_RS25120 (LGM22_25120)	13634..14911	+	1278	WP_009309915.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..283582	283582

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11571.24 Da
• Isoelectric Point: 6.4661

>T219435 WP_009309918.1 NZ_CP084807:c10590-10285 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVVPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T219435 NZ_CP116411:1081396-1081731 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8362.31 Da
• Isoelectric Point: 4.7405

>AT219435 WP_006788213.1 NZ_CP084807:c10810-10592 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT219435 NZ_CP116411:1081148-1081396 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A7H9GHC5

Antitoxin

• AlphaFold DB: A0A1D8K3R4