Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 55024..55760 Replicon plasmid pKqq_SB98_2
Accession NZ_CP084805
Organism Klebsiella quasipneumoniae subsp. quasipneumoniae strain SB98

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag LGM23_RS28025 Protein ID WP_003026803.1
Coordinates 55278..55760 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag LGM23_RS28020 Protein ID WP_003026799.1
Coordinates 55024..55290 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM23_RS27975 (LGM23_27975) 51086..51448 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
LGM23_RS27980 (LGM23_27980) 51498..51848 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
LGM23_RS27985 (LGM23_27985) 52206..52475 + 270 WP_004152102.1 hypothetical protein -
LGM23_RS27990 (LGM23_27990) 52463..53038 + 576 WP_004152103.1 hypothetical protein -
LGM23_RS27995 (LGM23_27995) 53069..53563 + 495 WP_016831217.1 DNA-binding protein -
LGM23_RS28000 (LGM23_28000) 53607..53975 + 369 WP_004152105.1 hypothetical protein -
LGM23_RS28005 (LGM23_28005) 54009..54212 + 204 WP_004152106.1 HHA domain-containing protein -
LGM23_RS28010 (LGM23_28010) 54261..54518 + 258 WP_004152107.1 hypothetical protein -
LGM23_RS28015 (LGM23_28015) 54594..54848 + 255 WP_004152108.1 hypothetical protein -
LGM23_RS28020 (LGM23_28020) 55024..55290 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
LGM23_RS28025 (LGM23_28025) 55278..55760 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
LGM23_RS28030 (LGM23_28030) 55919..57084 - 1166 Protein_59 IS3 family transposase -
LGM23_RS28035 (LGM23_28035) 57261..58223 - 963 WP_004152113.1 zinc metalloprotease HtpX -
LGM23_RS28040 (LGM23_28040) 58210..58698 - 489 WP_004152114.1 phosphate-starvation-inducible PsiE family protein -
LGM23_RS28045 (LGM23_28045) 58813..58959 - 147 Protein_62 diguanylate cyclase -
LGM23_RS28050 (LGM23_28050) 59197..59394 - 198 WP_004152115.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..195131 195131


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T219421 WP_003026803.1 NZ_CP084805:55278-55760 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T219421 NZ_CP116409:2747776-2749098 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGCGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT219421 WP_003026799.1 NZ_CP084805:55024-55290 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT219421 NZ_CP116409:2747510-2747776 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References