Detailed information of TA system TA219404

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 53667..54403
• Replicon: plasmid pKqs_09A323_1
• Accession: NZ_CP084784
• Organism: Klebsiella quasipneumoniae subsp. similipneumoniae strain 09A323

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: LGM26_RS25290
• Protein ID: WP_003026803.1
• Coordinates: 53921..54403 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: LGM26_RS25285
• Protein ID: WP_003026799.1
• Coordinates: 53667..53933 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM26_RS25240 (LGM26_25240)	49729..50091	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
LGM26_RS25245 (LGM26_25245)	50141..50491	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
LGM26_RS25250 (LGM26_25250)	50849..51118	+	270	WP_004152102.1	hypothetical protein	-
LGM26_RS25255 (LGM26_25255)	51106..51681	+	576	WP_004152103.1	hypothetical protein	-
LGM26_RS25260 (LGM26_25260)	51712..52206	+	495	WP_004152104.1	DNA-binding protein	-
LGM26_RS25265 (LGM26_25265)	52250..52618	+	369	WP_004152105.1	hypothetical protein	-
LGM26_RS25270 (LGM26_25270)	52652..52855	+	204	WP_004152106.1	HHA domain-containing protein	-
LGM26_RS25275 (LGM26_25275)	52904..53161	+	258	WP_004152107.1	hypothetical protein	-
LGM26_RS25280 (LGM26_25280)	53237..53491	+	255	WP_004152108.1	hypothetical protein	-
LGM26_RS25285 (LGM26_25285)	53667..53933	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
LGM26_RS25290 (LGM26_25290)	53921..54403	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
LGM26_RS25295 (LGM26_25295)	54615..55961	+	1347	WP_020314316.1	ISNCY family transposase	-
LGM26_RS26590	56122..56253	+	132	WP_004218042.1	hypothetical protein	-
LGM26_RS25300 (LGM26_25300)	56462..57627	-	1166	Protein_59	IS3 family transposase	-
LGM26_RS25305 (LGM26_25305)	57804..58766	-	963	WP_004152113.1	zinc metalloprotease HtpX	-
LGM26_RS25310 (LGM26_25310)	58753..59241	-	489	WP_004152114.1	phosphate-starvation-inducible PsiE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ant(3'')-Ia / qacE / sul1	-	1..195396	195396

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T219404 WP_003026803.1 NZ_CP084784:53921-54403 [Klebsiella quasipneumoniae subsp. similipneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T219404 NZ_CP116405:2713699-2713806 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT219404 WP_003026799.1 NZ_CP084784:53667-53933 [Klebsiella quasipneumoniae subsp. similipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT219404 NZ_CP116405:c2713652-2713586 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66