Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 54729..55465 Replicon plasmid pKqs_12A476_1
Accession NZ_CP084780
Organism Klebsiella quasipneumoniae subsp. similipneumoniae strain 12A476

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag LGM27_RS24975 Protein ID WP_003026803.1
Coordinates 54983..55465 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag LGM27_RS24970 Protein ID WP_003026799.1
Coordinates 54729..54995 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM27_RS24925 (LGM27_24925) 50791..51153 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
LGM27_RS24930 (LGM27_24930) 51203..51553 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
LGM27_RS24935 (LGM27_24935) 51911..52180 + 270 WP_004152102.1 hypothetical protein -
LGM27_RS24940 (LGM27_24940) 52168..52743 + 576 WP_004152103.1 hypothetical protein -
LGM27_RS24945 (LGM27_24945) 52774..53268 + 495 WP_004152104.1 DNA-binding protein -
LGM27_RS24950 (LGM27_24950) 53312..53680 + 369 WP_004152105.1 hypothetical protein -
LGM27_RS24955 (LGM27_24955) 53714..53917 + 204 WP_004152106.1 HHA domain-containing protein -
LGM27_RS24960 (LGM27_24960) 53966..54223 + 258 WP_004152107.1 hypothetical protein -
LGM27_RS24965 (LGM27_24965) 54299..54553 + 255 WP_004152108.1 hypothetical protein -
LGM27_RS24970 (LGM27_24970) 54729..54995 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
LGM27_RS24975 (LGM27_24975) 54983..55465 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
LGM27_RS24980 (LGM27_24980) 55666..57069 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
LGM27_RS24985 (LGM27_24985) 57098..57730 - 633 WP_001567369.1 hypothetical protein -
LGM27_RS24990 (LGM27_24990) 57956..59302 + 1347 WP_077253535.1 ISNCY family transposase -
LGM27_RS24995 (LGM27_24995) 59351..59746 + 396 WP_004143398.1 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA2 / qacE / sul1 - 1..239503 239503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T219390 WP_003026803.1 NZ_CP084780:54983-55465 [Klebsiella quasipneumoniae subsp. similipneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T219390 NZ_CP116404:35248-36600 [Burkholderia pseudomallei]
GTGAGCGCCCGCCGCGCACGCGCGACGCGCCTGCACCTGTGGATGAACGGCCTGCCCGTCGGCTACTGGGAGCACGCGCG
CGACGGCGAGCGCCTCGTCTACTTCGACGAATGGATCGGCGATCCGCAAGGCCGGCCGCTGTCGCTGTCGCTGCCGTTCA
CGCCGGGCAACCAGCCGTATCGCGGTCGGCTCGTCAGCGATTATTTCGACAACCTGCTGCCCGACAGCGAGCCGATCCGC
CGGCGAATCGCGATGCGCTACCGCACGGGCGGCACGTCCGCGTTCGCGCTGCTCGCGACGCTCGGCCGCGATTGCGTCGG
CGCGCTGCAGATGCTGCCGCCCGACGAAGCGCCGGACGACATCGAACGCATCCGCGGCCACGCGCTCGCCGACGCGGACA
TCGCGCGCCTGCTGCGCGAAGTCACGTCCGCGCCGCAGGCCGGCCGGCACGCGCCGCTCGACGATCTGCGCCTGTCGATC
GCCGGCGCGCAGGAGAAGACCGCGCTGCTGCGCCACCGCGGCCGCTGGCTGCTGCCCGAAGGGAGCACGCCGACCACGCA
CATCCTGAAGCTGCCGCTCGGGCTCGTCGGCAACCGGCGCGCCGACATGCGCACGTCGGTCGAGAACGAATGGCTGTGCG
CGCGGATCGTCGCCGCGTACGGGTTGCCCGTCGCGCGCTGCGACATCGCTCAGTTCGACGATCAGAAAGCGCTCGTCGTC
GAGCGCTTCGACCGCCGGCCGTCGCGCGACGCACGCTGGCTCCTGCGGCTGCCGCAGGAAGACATGTGCCAGGCAACCGG
CACGTCCGCGCTCGACAAATATCAGGCCGACGGCGGCCCCGGCATCGAGACGATCATGGAAGTGCTCGCCGGCTCCGAAC
ACGCGCGGGACGACCGCCGCGCGTTCTTCGCGGCGCAGATCGTGTTCTGGCTGCTCGCCGCGACCGACGGCCACGCGAAG
AACTTCAGCATCGCGCACCTGCCCGGCAACCGCTACCGTTCGACGCCGCTTTACGACGTGCTGTCCGCGCATCCGGTCAT
CGGCCGGGGCGCGAACCAGTTGCCCGCGCAGCGCGCGCGGCTCGCGATGGGCGTGCGCGGCAAGCACATCCACTATCCGC
TGCACCAGATCCGGCGGCGGCACTGGATCGCGCAGGGCCAGCGCGTCGGCTTCGCGCCCGCCGACGTCGACGCGCTGATC
GACACGCTGACCGCGCGCACCGCGGACGTCGTCGACGCGGTGTCGGCGCGGCTGCCGCGCGATTTTCCGCGCGACGTCGC
CGATGCGATCTTCAGCGGAATGCTCGGCCTGAGCGCAAGGCTCGCCGGCGACGCGGCCGCGCGCGCACCATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT219390 WP_003026799.1 NZ_CP084780:54729-54995 [Klebsiella quasipneumoniae subsp. similipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT219390 NZ_CP116404:34841-35251 [Burkholderia pseudomallei]
ATGGCCATCCTCATCGAGCACGAGATCAAGACACTCGGCCAGCTGCGGCCGATTCTGCGCGGCTTCCGCAAATCGGCCGG
ATTGACGCAGGCGATGCTCGCGAGCCGCCTCGGCGTCACGCAGCAGACCTACGCGCAGTTCGAGGCGAACCCGGCGTCGG
CGAGCGTCGAGCGGCTGTTCAAGGTGCTGCGCGCGCTCGACATCGAACTCACGCTCACGCTCACGCAGGTCTACGCCGCG
CCCGCGGGCAAGGACAAGGGCGAGGTTGCGAAGACGGCCGCAGGCGCGCGCGCGCGCGCGGGCGCGCGACGTGCCGTGCC
GCCCGCGTCCGCGCCCGCTCCGAGCGCCGCCGGGCGCGCGCCCCGCCCCGCCCGCAAGCGCGCCGCCCCGAAAAAGCGGG
AGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References