Detailed information of TA system TA219388

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 4481111..4481814
• Replicon: chromosome
• Accession: NZ_CP084779
• Organism: Klebsiella quasipneumoniae subsp. similipneumoniae strain 12A476

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: LGM27_RS21755
• Protein ID: WP_049017155.1
• Coordinates: 4481111..4481452 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: LGM27_RS21760
• Protein ID: WP_074377667.1
• Coordinates: 4481473..4481814 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM27_RS21740 (4477838)	4477838..4478476	-	639	WP_023313890.1	HNH endonuclease	-
LGM27_RS21745 (4478656)	4478656..4479525	+	870	WP_064183904.1	HNH endonuclease	-
LGM27_RS21750 (4479845)	4479845..4480852	-	1008	WP_049017154.1	restriction endonuclease	-
LGM27_RS21755 (4481111)	4481111..4481452	-	342	WP_049017155.1	TA system toxin CbtA family protein	Toxin
LGM27_RS21760 (4481473)	4481473..4481814	-	342	WP_074377667.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LGM27_RS21765 (4481825)	4481825..4482367	-	543	WP_049017157.1	DNA repair protein RadC	-
LGM27_RS21770 (4482380)	4482380..4482823	-	444	WP_047365977.1	antirestriction protein	-
LGM27_RS21775 (4482854)	4482854..4483675	-	822	WP_064183906.1	DUF932 domain-containing protein	-
LGM27_RS21780 (4483795)	4483795..4484268	-	474	WP_064183907.1	hypothetical protein	-
LGM27_RS21785 (4484340)	4484340..4484792	-	453	WP_032410767.1	hypothetical protein	-
LGM27_RS21790 (4484828)	4484828..4485544	-	717	WP_074377668.1	WYL domain-containing protein	-
LGM27_RS21795 (4485788)	4485788..4486663	-	876	WP_064183909.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4481111..4510429	29318

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (13-111)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12724.66 Da
• Isoelectric Point: 6.4514

>T219388 WP_049017155.1 NZ_CP084779:c4481452-4481111 [Klebsiella quasipneumoniae subsp. similipneumoniae]
MKTLPATTPQAATLCLSPVAVWQMLLACLLEQHYGLTLNDTPFSDETVIQEHIDAGITLADAVNFLVEKYELVRIDRRGF
NWQEQSPYLRAVDILRARQATGLLKRNRISAAQ

Nucleotide

• Length: 342 bp

>T219388 NZ_CP116398:c3434749-3434444 [Methylomonas sp. EFPC3]
ATGAATGAAAAAATCAAAATCGCCGACTTACCCGTGTTTGACATTACCGAACATCTGGATAGCGATCAAGCCATTGCCGA
ATACCTGACCGTGGTTCTGGAAGAAGATGACCCGGCGGCCTTGGCTCACGCACTCGGCAGCATTGCCAGAGCGCGAGGCA
TGACCGAAATTGCCAATGCCGCCGGCATCAGCCGCGAGGCTTTGTATAAAGCCTTGCGTGCCGACGCCCAACCCCGCTTC
GAGACTATCGCCAAAGTTTGTAAGGCGCTTGGGGTTAAGTTGACGGTGCAGACGAGAGCGGCTTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12787.52 Da
• Isoelectric Point: 6.4714

>AT219388 WP_074377667.1 NZ_CP084779:c4481814-4481473 [Klebsiella quasipneumoniae subsp. similipneumoniae]
MKYTDSENDSFLKWGLNRNVTPCFVARLVQEGNRLHYLADRASITGKFSEAECLKLDVAFPHFISQMESMLTTGEMNPCH
AHCVTLYHNGFTCEADTLGSCGYVYIAVYPTHR

Nucleotide

• Length: 342 bp

>AT219388 NZ_CP116398:c3435049-3434753 [Methylomonas sp. EFPC3]
ATGTACACAATCAAACAACTCCCCGCATTCAGCGAATGGCTGTTTGGCTTGAAAGATGGATTGACGCACCGCCGGTTGGC
GCGCCGACTGGAAAAAGCACAACAAGGCAATCTCGGCGACGTGAAAGCGGTAGGCGAGGGTGTTTTTGAAATGCGTGAAC
ATTTCGGTCCCGGCTGGCGAATGTACTACGTGCAGCGGGGCGAGGTGTTGATTGTGATGCTGGGCGGCGGCGATAAATCT
AGTCAGGCTGCCGATATTGCCAAAGCTCAGTGCCTAGCCGCGTTGTTGGAGGACTGA