Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3845855..3846474 Replicon chromosome
Accession NZ_CP084779
Organism Klebsiella quasipneumoniae subsp. similipneumoniae strain 12A476

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LGM27_RS18710 Protein ID WP_002892050.1
Coordinates 3846256..3846474 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LGM27_RS18705 Protein ID WP_002892066.1
Coordinates 3845855..3846229 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM27_RS18695 (3841008) 3841008..3842201 + 1194 WP_004204747.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LGM27_RS18700 (3842224) 3842224..3845370 + 3147 WP_004204748.1 multidrug efflux RND transporter permease subunit AcrB -
LGM27_RS18705 (3845855) 3845855..3846229 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LGM27_RS18710 (3846256) 3846256..3846474 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LGM27_RS18715 (3846633) 3846633..3847199 + 567 WP_004204750.1 maltose O-acetyltransferase -
LGM27_RS18720 (3847171) 3847171..3847293 - 123 WP_032431198.1 hypothetical protein -
LGM27_RS18725 (3847336) 3847336..3847806 + 471 WP_004204751.1 YlaC family protein -
LGM27_RS18730 (3847775) 3847775..3849232 - 1458 WP_049117056.1 PLP-dependent aminotransferase family protein -
LGM27_RS18735 (3849333) 3849333..3850031 + 699 WP_004204753.1 GNAT family protein -
LGM27_RS18740 (3850028) 3850028..3850168 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LGM27_RS18745 (3850168) 3850168..3850431 - 264 WP_004204754.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T219387 WP_002892050.1 NZ_CP084779:3846256-3846474 [Klebsiella quasipneumoniae subsp. similipneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T219387 NZ_CP116398:c1953098-1952847 [Methylomonas sp. EFPC3]
ATGGACGCCATCAGTTACAGCGCCGCTCGCGCCAATTTGGCTAAAACCATGGACAAGGTTTGCGCCGACCATGCCCCCAT
CATCATCACCCGCAAAAGCGAATCCCCGGTGGTAATGCTGTCTCTGGAAGACTACCAAGCCATGGAGGAAACCGCCTACC
TGCTACGTTCCCCAGCTAACGCCCGCCGCTTGCTGGAATCGATCGCCGAGCTGGAATCCGGCCAAGGCACGGAGCGCGAA
TTAATCGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT219387 WP_002892066.1 NZ_CP084779:3845855-3846229 [Klebsiella quasipneumoniae subsp. similipneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT219387 NZ_CP116398:c1952850-1952590 [Methylomonas sp. EFPC3]
GTGAAAATCACTTTTTCCTCCGACGCCTGGGATAGCTATCTCTACTGGCAAGCCACCGACAAGGCCATTCTCAAACGTAT
CAACCAATTGATCAAAGAAATCCAGCGCCAGCCCTTCGAGGGCATCGGTAAGCCGGAACCTTTGCAACACGGTTTGTCCG
GCTATTGGTCGCGGCGAATTAACGACGAGCATCGAATCGTTTATAAAGTTGCCGGGGATACCTTGTTGATTGCGCAGTTG
CGGTTTCATTATGAGTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References