Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 121713..122452 Replicon plasmid pKqs_CIP110288_1
Accession NZ_CP084778
Organism Klebsiella quasipneumoniae subsp. similipneumoniae strain CIP110288

Toxin (Protein)


Gene name tacT Uniprot ID A0A5E1AVR5
Locus tag LGM28_RS25910 Protein ID WP_013087172.1
Coordinates 121713..122198 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A5E1AWX8
Locus tag LGM28_RS25915 Protein ID WP_012540086.1
Coordinates 122186..122452 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM28_RS25850 (LGM28_25850) 117094..117507 - 414 WP_136084777.1 helix-turn-helix domain-containing protein -
LGM28_RS25855 (LGM28_25855) 117508..117786 - 279 WP_004152721.1 helix-turn-helix transcriptional regulator -
LGM28_RS25860 (LGM28_25860) 117776..118096 - 321 WP_004152720.1 type II toxin-antitoxin system RelE/ParE family toxin -
LGM28_RS25865 (LGM28_25865) 118177..118401 - 225 WP_063106387.1 hypothetical protein -
LGM28_RS25870 (LGM28_25870) 118412..118624 - 213 WP_063106386.1 hypothetical protein -
LGM28_RS25875 (LGM28_25875) 118685..119014 - 330 WP_162544171.1 hypothetical protein -
LGM28_RS25880 (LGM28_25880) 119001..119246 - 246 WP_074155869.1 hypothetical protein -
LGM28_RS25885 (LGM28_25885) 119885..120202 - 318 WP_038807150.1 hypothetical protein -
LGM28_RS25890 (LGM28_25890) 120217..120567 - 351 WP_063106385.1 hypothetical protein -
LGM28_RS25895 (LGM28_25895) 120564..120833 - 270 WP_038807148.1 hypothetical protein -
LGM28_RS25900 (LGM28_25900) 121211..121330 - 120 WP_223230170.1 Hok/Gef family protein -
LGM28_RS25905 (LGM28_25905) 121278..121430 - 153 Protein_121 DUF5431 family protein -
LGM28_RS25910 (LGM28_25910) 121713..122198 - 486 WP_013087172.1 GNAT family N-acetyltransferase Toxin
LGM28_RS25915 (LGM28_25915) 122186..122452 - 267 WP_012540086.1 DUF1778 domain-containing protein Antitoxin
LGM28_RS25920 (LGM28_25920) 122752..123075 + 324 WP_012540250.1 metalloregulator ArsR/SmtB family transcription factor -
LGM28_RS25925 (LGM28_25925) 123077..123790 + 714 WP_012540184.1 arsenical resistance protein ArsH -
LGM28_RS25930 (LGM28_25930) 123799..124344 + 546 WP_012540227.1 sigma-70 family RNA polymerase sigma factor -
LGM28_RS25935 (LGM28_25935) 124420..124782 + 363 WP_012540155.1 arsenic metallochaperone ArsD family protein -
LGM28_RS25940 (LGM28_25940) 124809..126560 + 1752 WP_162866166.1 arsenical pump-driving ATPase -
LGM28_RS25945 (LGM28_25945) 126685..127221 + 537 WP_012540238.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..151441 151441


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(27-152)

Antitoxin

Vibrio phage ICP1, Orf50

(2-87)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17810.62 Da        Isoelectric Point: 9.8560

>T219375 WP_013087172.1 NZ_CP084778:c122198-121713 [Klebsiella quasipneumoniae subsp. similipneumoniae]
VGRVTVPEPLSSFHQVAEFVCGETVLDDWLKQKGLKNQALGSARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Download         Length: 486 bp

>T219375 NZ_CP116390:2130421-2130912 [Bifidobacterium longum]
ATGAGGCAGTCAAAGCAGGTGTCGATCTTGTGCGACACCAATATCCTTATTTATTACATGCTCTGTACGCCTCGGTTCAT
CAAGGATGTCATGAAACTGTTCGAGCGATGCGTCGAGCGGGAGGTAAAGCTGTCCTGTGCGTCACTGTCGCTGAAGGACA
TGTTCTACATCACCCCGGAACGATTCAAAGAACGATTCATGAATGACGAATCCATGGTCGTTGCGACCACCGCCAAGCTG
ATATTGCCGGAGGTGGCATGGTCGGGAATCAGCAAACTCAAGGAGCTCATGACAATCGTGCCGATTGGCGCGGAGGAATG
TGAATTGGCGCAGTCGTTGCGGCACGCGCATAACGATTTCGAGGATGACTTTATTATCGCCGCCGCACAACAATCCGGGG
CCACCTATGTGGTGACGTATGACAAAAAACTAGCCAAGCATTTTCCCGAATATTGCAAAACGCCGGCTGAAATCGTGGCG
GCGCTGGCATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10076.47 Da        Isoelectric Point: 4.3721

>AT219375 WP_012540086.1 NZ_CP084778:c122452-122186 [Klebsiella quasipneumoniae subsp. similipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV

Download         Length: 267 bp

>AT219375 NZ_CP116390:2130032-2130424 [Bifidobacterium longum]
ATGACCATGGTCCAAATGAACGTTCGCATTGATGCGAAACTGAAGAACGAGGTCGAAGAAGTCCTCAAGGGCAAAGGCGT
ATCGGTGAGTGACGTAATCCGGTCGCTGTGGTTGTACATCGCCGACCGCAAGGAAGTGCCGGCATTGGAAACGACCGCCG
AAGAGCAGGCCCGCGAGGCGGAAAAGCAGCGCAAGCTGAAACTGATTCGAGAAGGTGCCGGATACGTGCACAAGGAACTG
GTCAAGGCCGGACTGATCTCAGAAGACACCGACCTCATGGAAGGCCTTACCTACAAGCAGTTCCGCGATGAGATAGCTGA
CGAACGGCTGAAGGAATACAGGCATCAGTCGGACTATGCGCGTTATTTGGAGAAGGAGCGGAGTCTGTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1AVR5

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1AWX8

References