Detailed information of TA system TA219374

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 26803..27539
• Replicon: plasmid pKqs_CIP110288_1
• Accession: NZ_CP084778
• Organism: Klebsiella quasipneumoniae subsp. similipneumoniae strain CIP110288

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A142I508
• Locus tag: LGM28_RS25415
• Protein ID: WP_032720647.1
• Coordinates: 27057..27539 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: LGM28_RS25410
• Protein ID: WP_063106370.1
• Coordinates: 26803..27069 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM28_RS25395 (LGM28_25395)	23206..24372	+	1167	WP_136084789.1	hypothetical protein	-
LGM28_RS25400 (LGM28_25400)	25092..26374	+	1283	Protein_22	IS3 family transposase	-
LGM28_RS25405 (LGM28_25405)	26359..26496	+	138	WP_226648960.1	cell envelope integrity TolA C-terminal domain-containing protein	-
LGM28_RS25410 (LGM28_25410)	26803..27069	+	267	WP_063106370.1	DUF1778 domain-containing protein	Antitoxin
LGM28_RS25415 (LGM28_25415)	27057..27539	+	483	WP_032720647.1	GNAT family N-acetyltransferase	Toxin
LGM28_RS25420 (LGM28_25420)	27755..29155	+	1401	WP_063106369.1	ISNCY family transposase	-
LGM28_RS25425 (LGM28_25425)	29343..29762	+	420	WP_012540023.1	ASCH domain-containing protein	-
LGM28_RS25430 (LGM28_25430)	30344..31345	+	1002	WP_040107896.1	LacI family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..151441	151441
	-	inside	IScluster/Tn	-	-	22373..29155	6782

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17335.02 Da
• Isoelectric Point: 9.8720

>T219374 WP_032720647.1 NZ_CP084778:27057-27539 [Klebsiella quasipneumoniae subsp. similipneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T219374 NZ_CP116389:2762-3049 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10151.58 Da
• Isoelectric Point: 4.5256

>AT219374 WP_063106370.1 NZ_CP084778:26803-27069 [Klebsiella quasipneumoniae subsp. similipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDETAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT219374 NZ_CP116389:3036-3350 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACACAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A142I508