Detailed information of TA system TA219371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2529965..2531553
• Replicon: chromosome
• Accession: NZ_CP084777
• Organism: Klebsiella quasipneumoniae subsp. similipneumoniae strain CIP110288

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LGM28_RS12275
• Protein ID: WP_004205919.1
• Coordinates: 2529965..2531287 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A2A5MFC9
• Locus tag: LGM28_RS12280
• Protein ID: WP_004205921.1
• Coordinates: 2531287..2531553 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM28_RS12240 (2525047)	2525047..2525265	+	219	WP_002904808.1	ParD-like family protein	-
LGM28_RS12245 (2525246)	2525246..2526028	+	783	WP_023289670.1	type I methionyl aminopeptidase	-
LGM28_RS12250 (2526297)	2526297..2527412	+	1116	WP_004205914.1	serine hydrolase domain-containing protein	-
LGM28_RS12255 (2527399)	2527399..2527743	+	345	WP_004205915.1	hypothetical protein	-
LGM28_RS12260 (2527772)	2527772..2528305	+	534	WP_004205916.1	hypothetical protein	-
LGM28_RS12265 (2528308)	2528308..2528514	+	207	WP_004205917.1	helix-turn-helix transcriptional regulator	-
LGM28_RS12270 (2528656)	2528656..2529948	+	1293	WP_004205918.1	glycoside hydrolase family 10 protein	-
LGM28_RS12275 (2529965)	2529965..2531287	-	1323	WP_004205919.1	type II toxin-antitoxin system HipA family toxin	Toxin
LGM28_RS12280 (2531287)	2531287..2531553	-	267	WP_004205921.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LGM28_RS12285 (2531836)	2531836..2532162	-	327	WP_017900379.1	hydroxyisourate hydrolase	-
LGM28_RS12290 (2532159)	2532159..2532659	-	501	WP_080846687.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
LGM28_RS12295 (2532637)	2532637..2534016	-	1380	WP_136085014.1	nucleobase:cation symporter-2 family protein	-
LGM28_RS12300 (2534362)	2534362..2535516	+	1155	WP_004205928.1	FAD-dependent urate hydroxylase HpxO	-
LGM28_RS12305 (2535497)	2535497..2536420	-	924	WP_004205929.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49013.27 Da
• Isoelectric Point: 8.2655

>T219371 WP_004205919.1 NZ_CP084777:c2531287-2529965 [Klebsiella quasipneumoniae subsp. similipneumoniae]
MATLTTWMNNARVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRERIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLAALRWQTLDEAQLTALLTAYQSDIPLGMVTGQDDFRISVAGAQEKTAL
LRMGEHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARALGLAVPEAEIINTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILGEFADRVP
QAIESARQTLPSDFSAHVWRAITENMLKLHARLQQGLQAG

Nucleotide

• Length: 1323 bp

>T219371 NZ_CP116384:1218728-1219663 [Vibrio sp. SCSIO 43137]
ATACCTAAACTAAAAAAGCAATTTCTTGAAGTATCAGATGCAATCGAATTAGGTAACCCGTCAATCACGGAAAAGGACTA
TTGGGTGGTATCCCTGCTTGCCATGTTAGAAAGTGTGGAAAGTGAACACCACCAACTTGTTTTTTCAGGTGGAACAGCTC
TGGCCAAGTCCAACATCAAGATTCTGCGCATGTCCGAAGACGTCGACATAAAATTAATTCCAAATCAGGAATTTCTCAAT
AAAGGTACAGGTGCAAAGAGAAAAGCTCGCAAGGCGTTAGTTGAGAACCTCATAGCAAGGTTGGTTGACCACGCTTATCT
GAAATATAGTAAAAGAACGGTACGCGATAGCTATCGCTATGTCGAAATCGAAATAGAGTACCCGCAACAATACCATCAAG
CACCATGTCTAAGGCCTTATATTAAACTGGAACTTATCGAGACCATTGAGTTTCAGGGCGTTGAACCTCGCTCTATATCA
TCGCTGGTTGCGCAGAGCTACAATCAACCTGCTGAAGTAAACGCAATGTCATGCATTTCCATTGATTTGACGTTGGTTGA
AAAAGTACTTTCTATGTTGCGCCGCACCATGTCAGTTAAGCGTAATCCACAGCGTAAGGATGACGAAACACTGGTTCGTC
ACATCTACGATGTTCACTGTATTAATGCCCAGCATACGATTGATATAGGCGCATTAAAGCCGATATTTGATACCGTTTTG
GAAGAGGACAAGGCGCGATATGGTAATCAACATCAAGACTTTGTCGCCGACCCGCGCAATGAATTGAAATTAGGATTAGA
AGAGCTAGAAACCAACGAGAAGTTCCGTGAACGTTTTGAAGCATTTGTAGCTCCAATGGTGTACAGCACAGAACCACATG
ACTTTGATACCTGTTTTACCTCATTCAAAAATATTTCAGAGCAACTTATCAACTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9940.38 Da
• Isoelectric Point: 7.1997

>AT219371 WP_004205921.1 NZ_CP084777:c2531553-2531287 [Klebsiella quasipneumoniae subsp. similipneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQEALASRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKPAETAMIP
DNTTDLDW

Nucleotide

• Length: 267 bp

>AT219371 NZ_CP116384:1218321-1218725 [Vibrio sp. SCSIO 43137]
ATGACTGCAACTGATCGAATTTATCAAAAGATAAAGCGCTCTCGCCGCTATGTGTTCGAACGTAAAGATTTCGACAACTA
TGCTAGCTATGACCAGATTGGACGCGTCTTAAAACAACTTGTAGATAAAGGCGTACTGATGAAAATCGGATATGGCTTAT
ACACCAAGTCAACAATCAACAGCCTAACCAATAAACCCATGCCTACGAACCCGGGTGGTACTGACGCAATCATGCGAGAG
GTTCTAAAAATGCGTGGTGTAGACTTTGAGCTGGATGCTATGTCATTGAAGAGCCTCAATGGACAGTCCACTCAAATACC
GGCATCCATCAGGTATAAGTGGAACTCCAAATATTTCAACCGCAGCCTTAAGGTCGGTAACCGAGTATTGAACGAGCGAC
AATGA

Structures

Antitoxin

• AlphaFold DB: A0A2A5MFC9