Detailed information of TA system TA219361

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 58808..59451
• Replicon: plasmid pKqs_SB610_6
• Accession: NZ_CP084776
• Organism: Klebsiella quasipneumoniae subsp. similipneumoniae strain SB610

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: LGM29_RS29595
• Protein ID: WP_220675994.1
• Coordinates: 59035..59451 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: LGM29_RS29590
• Protein ID: WP_001261276.1
• Coordinates: 58808..59038 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM29_RS29570 (LGM29_29570)	54965..55747	-	783	WP_048235058.1	site-specific integrase	-
LGM29_RS29575 (LGM29_29575)	55785..56501	-	717	WP_053059918.1	hypothetical protein	-
LGM29_RS29580 (LGM29_29580)	56494..57303	-	810	WP_053059919.1	hypothetical protein	-
LGM29_RS29585 (LGM29_29585)	57300..58220	-	921	WP_048235060.1	hypothetical protein	-
LGM29_RS29590 (LGM29_29590)	58808..59038	+	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LGM29_RS29595 (LGM29_29595)	59035..59451	+	417	WP_220675994.1	type II toxin-antitoxin system VapC family toxin	Toxin
LGM29_RS29600 (LGM29_29600)	59486..59731	+	246	WP_065905238.1	hypothetical protein	-
LGM29_RS29605 (LGM29_29605)	59795..60013	+	219	WP_021312527.1	hypothetical protein	-
LGM29_RS29610 (LGM29_29610)	60092..60418	+	327	WP_124989605.1	hypothetical protein	-
LGM29_RS29615 (LGM29_29615)	60658..60921	+	264	WP_004118691.1	hypothetical protein	-
LGM29_RS29620 (LGM29_29620)	60918..61484	+	567	WP_009654312.1	hypothetical protein	-
LGM29_RS29625 (LGM29_29625)	61515..62009	+	495	WP_016528789.1	hypothetical protein	-
LGM29_RS29630 (LGM29_29630)	62070..62273	+	204	WP_004150739.1	HHA domain-containing protein	-
LGM29_RS29635 (LGM29_29635)	62322..62501	+	180	WP_226550274.1	hypothetical protein	-
LGM29_RS29640 (LGM29_29640)	62535..63232	+	698	WP_108702448.1	IS1 family transposase	-
LGM29_RS29645 (LGM29_29645)	63406..63729	+	324	WP_017899891.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..84354	84354

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15138.59 Da
• Isoelectric Point: 7.8637

>T219361 WP_220675994.1 NZ_CP084776:59035-59451 [Klebsiella quasipneumoniae subsp. similipneumoniae]
MKKTWMLDTNICSFIMREQPESVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T219361 NZ_CP116348:c212166-210841 [Enterobacter ludwigii]
ATGAGCCGGAAACAGCAGCGTCTGGCAATCTGGATGAACGGAATCAAAGTCGGATACTGGGAAAAAAGTAAAGGGGTAGA
CAGCTTACAATACCTTCCTGACTGGAGTGCAGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTCCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTTCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGATGAACCCACAGATTTATATTCCGTGAAGTATCACCCGCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAACGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGATCGGTGGTGTATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCGCTCGGACTTGTGGGGAACATGAAAGCCGACATGCGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGAACAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAAACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCATTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAAGTTCTGAGTAATTCAGACCGTGCTGAGC
GTGACAAAGCGCAGTTTTTTATGACGCAGATAGTTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCACTCTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCCTGGCAGAAGTGCAAACTGGCGATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACAGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCGGGTGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCAGCAGCTTGCGGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTGGCTGAGAAATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT219361 WP_001261276.1 NZ_CP084776:58808-59038 [Klebsiella quasipneumoniae subsp. similipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT219361 NZ_CP116348:c212464-212171 [Enterobacter ludwigii]
ATCAATAATGATTACCCGTTAAACACGCTCAACCAGTTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGGGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCTATCCTGGGCGTAAAAATCAGCTTCTCCTCGACAACCGCCTCTTCAGAGGGGAAG
CAGACGGAAGAAATGCTTAAATCAAACTCACCTGCACGACAGGAGAAATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A387K023