Detailed information of TA system TA219360

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 8621..9357
• Replicon: plasmid pKqs_SB610_6
• Accession: NZ_CP084776
• Organism: Klebsiella quasipneumoniae subsp. similipneumoniae strain SB610

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: LGM29_RS29300
• Protein ID: WP_003026803.1
• Coordinates: 8875..9357 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: LGM29_RS29295
• Protein ID: WP_003026799.1
• Coordinates: 8621..8887 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM29_RS29270 (LGM29_29270)	3640..4632	-	993	WP_001145103.1	hypothetical protein	-
LGM29_RS29275 (LGM29_29275)	4977..5681	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LGM29_RS29280 (LGM29_29280)	5833..7044	+	1212	WP_032415728.1	hypothetical protein	-
LGM29_RS29285 (LGM29_29285)	7178..7669	+	492	WP_004206662.1	hypothetical protein	-
LGM29_RS29290 (LGM29_29290)	7730..7933	+	204	WP_004206661.1	HHA domain-containing protein	-
LGM29_RS29295 (LGM29_29295)	8621..8887	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
LGM29_RS29300 (LGM29_29300)	8875..9357	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
LGM29_RS29305 (LGM29_29305)	9558..10961	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
LGM29_RS29310 (LGM29_29310)	10990..11622	-	633	WP_001567369.1	hypothetical protein	-
LGM29_RS29315 (LGM29_29315)	11887..12492	-	606	WP_000509966.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..84354	84354
	-	inside	IScluster/Tn	-	-	4977..22757	17780

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T219360 WP_003026803.1 NZ_CP084776:8875-9357 [Klebsiella quasipneumoniae subsp. similipneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T219360 NZ_CP116348:149591-150076 [Enterobacter ludwigii]
GTGGGACGTGTAACAGCACCGGAACCTTTATCCAGCTCACATCAAATGGCTGAGTTCGTAAGCGGGGAGGCCGTTCTCGA
TGACTGGCTTAAACAGCGAGGGTTGAAAAATCAGGCACTTGGTGCTGCCAGAACGTTCGTTGTATGTAAAACAGGCACCA
AACAGGTTGCCGGTTTTTACTCCCTGGCCACCGGCAGCGTTAACCATGTAGAAGCAACGGGAAGTCTCAGGCGCAATATG
CCAGATCCAATCCCGGTAATCATACTTGCTCGACTGGCTATCGATGCCTCATTTCGTGGACAAGGGCTGGGTGCTGATTT
ACTGCACGATGCAGTATTGCGCTGTTATCGTGTGGCTGAAAATATAGGCGTTCGTGCAATTATGGTTCATGCGCTGACAG
AAGAAGCCAAAGGATTTTATCTTCATCATGGTTTTAAGGCATCGCAAACCCAAGAACGGACTCTGTTTCTCAAACTGCCG
CAATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT219360 WP_003026799.1 NZ_CP084776:8621-8887 [Klebsiella quasipneumoniae subsp. similipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT219360 NZ_CP116348:149337-149603 [Enterobacter ludwigii]
ATGAAATCAGATGTCCAGCTCAACCTCAGAGCCAAGGAGTCTCAAAGAGCACTTATCGATGCTGCTGCAGAAATCCTTCA
TAAATCACGCACAGACTTTATCCTTGAAATGGCGTGCCAGGCTGCTGAGAATGTGATCCTCGATCGTCGCGTATTCAACT
TTAACGATGAGCAGTATGCTGAGTTTATCGATATGCTCGATGCGCCAGTTGCGGATGACGCTGCCATCAATAAACTCCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66