Detailed information of TA system TA219343

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 5000222..5000879
• Replicon: chromosome
• Accession: NZ_CP084767
• Organism: Klebsiella variicola subsp. tropica strain CDC4241-71

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: W8UCT0
• Locus tag: LGN97_RS24025
• Protein ID: WP_002916310.1
• Coordinates: 5000222..5000632 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: LGN97_RS24030
• Protein ID: WP_043520925.1
• Coordinates: 5000613..5000879 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGN97_RS24005 (LGN97_24005)	4996222..4997955	-	1734	WP_136086359.1	single-stranded-DNA-specific exonuclease RecJ	-
LGN97_RS24010 (LGN97_24010)	4997961..4998674	-	714	WP_061153358.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
LGN97_RS24015 (LGN97_24015)	4998697..4999593	-	897	WP_043876074.1	site-specific tyrosine recombinase XerD	-
LGN97_RS24020 (LGN97_24020)	4999694..5000215	+	522	WP_008806427.1	flavodoxin FldB	-
LGN97_RS24025 (LGN97_24025)	5000222..5000632	-	411	WP_002916310.1	protein YgfX	Toxin
LGN97_RS24030 (LGN97_24030)	5000613..5000879	-	267	WP_043520925.1	FAD assembly factor SdhE	Antitoxin
LGN97_RS24035 (LGN97_24035)	5001125..5002108	+	984	WP_136086358.1	tRNA-modifying protein YgfZ	-
LGN97_RS24040 (LGN97_24040)	5002286..5002945	-	660	WP_061153356.1	hemolysin III family protein	-
LGN97_RS24045 (LGN97_24045)	5003109..5003420	-	312	WP_074387613.1	N(4)-acetylcytidine aminohydrolase	-
LGN97_RS24050 (LGN97_24050)	5003471..5004199	+	729	WP_136086403.1	MurR/RpiR family transcriptional regulator	-
LGN97_RS24055 (LGN97_24055)	5004319..5005752	+	1434	WP_074387612.1	6-phospho-beta-glucosidase BglA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16049.85 Da
• Isoelectric Point: 11.4778

>T219343 WP_002916310.1 NZ_CP084767:c5000632-5000222 [Klebsiella variicola subsp. tropica]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Nucleotide

• Length: 411 bp

>T219343 NZ_CP116313:c179873-179523 [Bacillus thuringiensis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10511.01 Da
• Isoelectric Point: 5.1116

>AT219343 WP_043520925.1 NZ_CP084767:c5000879-5000613 [Klebsiella variicola subsp. tropica]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDTDKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT219343 NZ_CP116313:c180165-179878 [Bacillus thuringiensis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Structures

Toxin

• AlphaFold DB: A0A0H3GSW7