Detailed information of TA system TA219330

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1615953..1616572
• Replicon: chromosome
• Accession: NZ_CP084767
• Organism: Klebsiella variicola subsp. tropica strain CDC4241-71

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: LGN97_RS07645
• Protein ID: WP_002892050.1
• Coordinates: 1615953..1616171 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: LGN97_RS07650
• Protein ID: WP_074386716.1
• Coordinates: 1616198..1616572 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGN97_RS07610 (LGN97_07610)	1611997..1612260	+	264	WP_074386720.1	type B 50S ribosomal protein L31	-
LGN97_RS07615 (LGN97_07615)	1612260..1612400	+	141	WP_002892018.1	type B 50S ribosomal protein L36	-
LGN97_RS07620 (LGN97_07620)	1612397..1613095	-	699	WP_074386719.1	GNAT family protein	-
LGN97_RS07625 (LGN97_07625)	1613196..1614653	+	1458	WP_074386718.1	PLP-dependent aminotransferase family protein	-
LGN97_RS07630 (LGN97_07630)	1614622..1615092	-	471	WP_061155526.1	YlaC family protein	-
LGN97_RS07635 (LGN97_07635)	1615126..1615257	+	132	Protein_1477	hypothetical protein	-
LGN97_RS07640 (LGN97_07640)	1615229..1615795	-	567	WP_136050242.1	maltose O-acetyltransferase	-
LGN97_RS07645 (LGN97_07645)	1615953..1616171	-	219	WP_002892050.1	HHA domain-containing protein	Toxin
LGN97_RS07650 (LGN97_07650)	1616198..1616572	-	375	WP_074386716.1	Hha toxicity modulator TomB	Antitoxin
LGN97_RS07655 (LGN97_07655)	1617054..1620200	-	3147	WP_074386715.1	multidrug efflux RND transporter permease subunit AcrB	-
LGN97_RS07660 (LGN97_07660)	1620223..1621416	-	1194	WP_074386714.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T219330 WP_002892050.1 NZ_CP084767:c1616171-1615953 [Klebsiella variicola subsp. tropica]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T219330 NZ_CP116303:1734689-1734973 [Tenacibaculum finnmarkense]
ATGGGAACTTCAAGATTTGAAATAGCTGATTATTTGGAAAGCAAGGAAATGATTGCCGAATACCTTAACACAGTTTTGGA
AGAAGGCAATAATGCAGATGTAATTAATGCAATCGGGAATATTGCTAAAGCTATCGGAATGACAAAAATTGCAGAAGAAA
CTGGTTTAAGCAGACCGAGTTTGTACAAAGCGTTATCAGATGGAGCGAAACCTCAATTTGCAACAATTATGAAAGTATTA
AAAGCTATCGGAGGACAAATACAAGTAAATCCAGCATCTGCCTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14354.03 Da
• Isoelectric Point: 4.8989

>AT219330 WP_074386716.1 NZ_CP084767:c1616572-1616198 [Klebsiella variicola subsp. tropica]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSADLQKWKKSGNRLFRCFVNASRENPASLSC

Nucleotide

• Length: 375 bp

>AT219330 NZ_CP116303:1734386-1734685 [Tenacibaculum finnmarkense]
ATGTTCTTTATTGAGAAAACAACCGAATTTGACAAATGGCTGAAAAAGTTGAAAGACTTCAAAGCAAAAGCTAAAATTTT
GTTCAGAATTCAAAAATTGGAATCTGACGAACATTTTGGAGATTGTAAAACTGTTGGTGATGGAATTCGGGAAATGCGAA
TAAATTTTGCAAAAGGTTACAGAATTTACTTCAAGGAAAAAGATGGAAAAATAATTATTCTGTTAATTGGTGGAGATAAA
TCAACACAACAAAAGGATATTACGAAAGCGAAAGAAATTTGGAAAAAATTAAATAAATAA

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2