Detailed information of TA system TA219329

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 1000509..1001085
• Replicon: chromosome
• Accession: NZ_CP084767
• Organism: Klebsiella variicola subsp. tropica strain CDC4241-71

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: LGN97_RS04720
• Protein ID: WP_012542979.1
• Coordinates: 1000509..1000796 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: LGN97_RS04725
• Protein ID: WP_136086508.1
• Coordinates: 1000783..1001085 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGN97_RS04690 (LGN97_04690)	996086..996604	-	519	WP_100730524.1	FidL-like protein	-
LGN97_RS04695 (LGN97_04695)	996601..997404	-	804	WP_074387176.1	winged helix-turn-helix domain-containing protein	-
LGN97_RS04700 (LGN97_04700)	997426..998010	-	585	WP_064180527.1	TetR/AcrR family transcriptional regulator	-
LGN97_RS04705 (LGN97_04705)	998152..998934	-	783	WP_074387175.1	NAD(P)H-dependent oxidoreductase	-
LGN97_RS04710 (LGN97_04710)	999021..999929	+	909	WP_074387174.1	LysR family transcriptional regulator	-
LGN97_RS04715 (LGN97_04715)	999923..1000342	+	420	WP_100676438.1	FosA5 family fosfomycin resistance glutathione transferase	-
LGN97_RS04720 (LGN97_04720)	1000509..1000796	+	288	WP_012542979.1	BrnT family toxin	Toxin
LGN97_RS04725 (LGN97_04725)	1000783..1001085	+	303	WP_136086508.1	BrnA antitoxin family protein	Antitoxin
LGN97_RS04730 (LGN97_04730)	1001165..1002577	-	1413	WP_061155894.1	PLP-dependent aminotransferase family protein	-
LGN97_RS04735 (LGN97_04735)	1002755..1002919	+	165	WP_008807599.1	DUF1127 domain-containing protein	-
LGN97_RS04740 (LGN97_04740)	1003085..1003549	+	465	WP_074387171.1	MarR family transcriptional regulator	-
LGN97_RS04745 (LGN97_04745)	1003556..1004623	+	1068	WP_061155892.1	HlyD family secretion protein	-
LGN97_RS04750 (LGN97_04750)	1004613..1005677	+	1065	WP_136086507.1	DUF2955 domain-containing protein	-
LGN97_RS04755 (LGN97_04755)	1005674..1005928	-	255	WP_061155890.1	DUF3343 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11245.77 Da
• Isoelectric Point: 8.6574

>T219329 WP_012542979.1 NZ_CP084767:1000509-1000796 [Klebsiella variicola subsp. tropica]
MPMEFEWDANKALSNLRKHGVRFEEAVLVFDDPRHLSRQERFENGEYRWQTIGLVHGILVILVAHSVRFESGTEVIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T219329 NZ_CP116303:1734386-1734685 [Tenacibaculum finnmarkense]
ATGTTCTTTATTGAGAAAACAACCGAATTTGACAAATGGCTGAAAAAGTTGAAAGACTTCAAAGCAAAAGCTAAAATTTT
GTTCAGAATTCAAAAATTGGAATCTGACGAACATTTTGGAGATTGTAAAACTGTTGGTGATGGAATTCGGGAAATGCGAA
TAAATTTTGCAAAAGGTTACAGAATTTACTTCAAGGAAAAAGATGGAAAAATAATTATTCTGTTAATTGGTGGAGATAAA
TCAACACAACAAAAGGATATTACGAAAGCGAAAGAAATTTGGAAAAAATTAAATAAATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11593.24 Da
• Isoelectric Point: 9.4429

>AT219329 WP_136086508.1 NZ_CP084767:1000783-1001085 [Klebsiella variicola subsp. tropica]
MSMVKHKRDNPVALTAERDAELKALAKKTDDEIDYSDIPTTEDEQWSDAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Nucleotide

• Length: 303 bp

>AT219329 NZ_CP116303:1734689-1734973 [Tenacibaculum finnmarkense]
ATGGGAACTTCAAGATTTGAAATAGCTGATTATTTGGAAAGCAAGGAAATGATTGCCGAATACCTTAACACAGTTTTGGA
AGAAGGCAATAATGCAGATGTAATTAATGCAATCGGGAATATTGCTAAAGCTATCGGAATGACAAAAATTGCAGAAGAAA
CTGGTTTAAGCAGACCGAGTTTGTACAAAGCGTTATCAGATGGAGCGAAACCTCAATTTGCAACAATTATGAAAGTATTA
AAAGCTATCGGAGGACAAATACAAGTAAATCCAGCATCTGCCTGA