Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5389764..5390389 Replicon chromosome
Accession NZ_CP084766
Organism Klebsiella variicola subsp. variicola strain 01A065

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag LGN95_RS26085 Protein ID WP_008807903.1
Coordinates 5389764..5390147 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LGN95_RS26090 Protein ID WP_004150355.1
Coordinates 5390147..5390389 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGN95_RS26070 (LGN95_26070) 5387130..5388032 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LGN95_RS26075 (LGN95_26075) 5388029..5388664 + 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
LGN95_RS26080 (LGN95_26080) 5388661..5389590 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LGN95_RS26085 (LGN95_26085) 5389764..5390147 - 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
LGN95_RS26090 (LGN95_26090) 5390147..5390389 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LGN95_RS26095 (LGN95_26095) 5390594..5391511 + 918 WP_023296845.1 alpha/beta hydrolase -
LGN95_RS26100 (LGN95_26100) 5391526..5392467 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
LGN95_RS26105 (LGN95_26105) 5392512..5392949 - 438 WP_012543288.1 D-aminoacyl-tRNA deacylase -
LGN95_RS26110 (LGN95_26110) 5392946..5393806 - 861 WP_008807907.1 virulence factor BrkB family protein -
LGN95_RS26115 (LGN95_26115) 5393800..5394399 - 600 WP_136047524.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T219326 WP_008807903.1 NZ_CP084766:c5390147-5389764 [Klebsiella variicola subsp. variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

>T219326 NZ_CP116297:c765877-765593 [Tenacibaculum finnmarkense]
ATGGAAACTTCAAGATTTGAAATAGCTGATTATTTGGAAAGCAAAGAAATGATTGCGGAATATCTTAACACAGTTTTGGA
AGAAGGTAATAATGCAGATGTAATTAATGCAATCGGGAATATTGCTAAAGCTATCGGGATGACAAAAATTGCAGAAGAAA
CAGGTTTAAGCAGACCGAGTTTGTACAAAGCGTTATCAGATGGAGCGAAACCTCAATTTGCAACAATTATGAAAGTATTA
AAAGCAATCGGAGGGCAAATACAAGTAAATCCAGCATCTGCCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT219326 WP_004150355.1 NZ_CP084766:c5390389-5390147 [Klebsiella variicola subsp. variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT219326 NZ_CP116297:c766204-765881 [Tenacibaculum finnmarkense]
TTGTATCCAAGTAGATACAGTATTATGTTCTTTATTGAGAAAACAACTGAATTTGACAAATGGCTGAAAAAGTTGAAAGA
CTTCAAAGCAAAAGCTAAAATTTTGTTCAGAATTCAAAAATTGGAATCTGACGAACATTTTGGAGATTGTAAAACTGTTG
GCGATGGAATTCGGGAAATGCGAATAAATTTTGCAAAAGGCTACAGAATTTACTTCAAAGAAAAAGATGGAAAAGTAATT
GTTCTGCTAATTGGTGGAGATAAATCAACACAACAAAAGGACATTACGAAAGCGAAAGAAATTTGGAAAAAATTAAATAA
ATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References