Detailed information of TA system TA219325

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 5066615..5068296
• Replicon: chromosome
• Accession: NZ_CP084766
• Organism: Klebsiella variicola subsp. variicola strain 01A065

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: -
• Locus tag: LGN95_RS24590
• Protein ID: WP_040971114.1
• Coordinates: 5066615..5067532 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A0A223UHG0
• Locus tag: LGN95_RS24595
• Protein ID: WP_040971113.1
• Coordinates: 5067538..5068296 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGN95_RS24570 (LGN95_24570)	5061921..5063441	+	1521	WP_063105928.1	sugar ABC transporter ATP-binding protein	-
LGN95_RS24575 (LGN95_24575)	5063438..5064436	+	999	WP_004206363.1	ribose ABC transporter permease	-
LGN95_RS24580 (LGN95_24580)	5064467..5065408	+	942	WP_012543155.1	ABC transporter substrate-binding protein	-
LGN95_RS24585 (LGN95_24585)	5065483..5066490	+	1008	WP_022065799.1	carbohydrate kinase family protein	-
LGN95_RS24590 (LGN95_24590)	5066615..5067532	-	918	WP_040971114.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LGN95_RS24595 (LGN95_24595)	5067538..5068296	-	759	WP_040971113.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
LGN95_RS24600 (LGN95_24600)	5068381..5069109	-	729	WP_008807258.1	response regulator transcription factor	-
LGN95_RS24605 (LGN95_24605)	5069382..5071529	+	2148	WP_077625434.1	formate dehydrogenase subunit alpha	-
LGN95_RS24610 (LGN95_24610)	5072059..5072745	+	687	WP_016160120.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-246)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34737.11 Da
• Isoelectric Point: 5.4707

>T219325 WP_040971114.1 NZ_CP084766:c5067532-5066615 [Klebsiella variicola subsp. variicola]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRTIALPHIRDALARIAANLQQQ
AGMNAVLQANRSDEMRVVVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELNRPIFNGFIAYLLSHNRPLAEVLSPRWKDIAEPFYREFSGMTFETIALEELTAVPYRMIDALKS
CFTQQDVDFLLSFKRGEPDWRLAPETRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T219325 NZ_CP116286:c186243-185944 [Pantoea stewartii]
ATGCAATTTGACGTTTACTCACACAAGAATGGTAAAAAATATCCTTATCTTGTTGAAATACAAAGCTACCTGATAGACAC
ACCAGGCCGTTTAATGGTTGCCCCCCTTGCTCTTTCGGGCGAGTTCGTGGGTTCAGGTGAACTTTATCCTGAGGTACTGG
TTAACGGGAGCAAGTACCGCGTTGTGACAACCGACATTGCGTCTGTCCCGGTGAAAGCGTTAGGGGATAAAGTGGGTGAT
GTCAGTCAGTATGAAGCAACAATAAAAAACGCCATTTTCAGGCTTTTTTGGGGTTTCTGA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28683.10 Da
• Isoelectric Point: 10.1988

>AT219325 WP_040971113.1 NZ_CP084766:c5068296-5067538 [Klebsiella variicola subsp. variicola]
MPSKLNWLLQNSAPGDVILQSWLSRHAISPSLAFKYTQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSMENKALLPKWFREFPGVEWIVISGQKLPVLDDKYRVMLDVKGKRLTGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQTKRLYLFFASFYEHGWLRRIDSQKIDLGAGKRQIVVNGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT219325 NZ_CP116286:c186464-186246 [Pantoea stewartii]
ATGAAATACAGAACTAACGTCACCATTGATAAAGAAGCCTACGAGATATTTTCATCTGCGGGTTTAAACCTTTCTAAAAT
GGTTAACGAGCTTGTCACTACTGAGGCTCGCAGAATCCGGGCTGAACGCTGGAAAGAAGAAAACCGTGAAAGCATGAAAG
CCTTCTCTGACTTTGTGGGAGAAGAGGGATCGCTTTCTGATATGTACAGGAACTGGTAG

Structures

Antitoxin

• AlphaFold DB: A0A223UHG0