Detailed information of TA system TA219324

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4777626..4778202
• Replicon: chromosome
• Accession: NZ_CP084766
• Organism: Klebsiella variicola subsp. variicola strain 01A065

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: LGN95_RS23240
• Protein ID: WP_012542979.1
• Coordinates: 4777915..4778202 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: LGN95_RS23235
• Protein ID: WP_022065028.1
• Coordinates: 4777626..4777928 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGN95_RS23210 (LGN95_23210)	4773034..4774098	-	1065	WP_008807596.1	DUF2955 domain-containing protein	-
LGN95_RS23215 (LGN95_23215)	4774088..4775155	-	1068	WP_008807597.1	HlyD family secretion protein	-
LGN95_RS23220 (LGN95_23220)	4775162..4775626	-	465	WP_008807598.1	MarR family transcriptional regulator	-
LGN95_RS23225 (LGN95_23225)	4775792..4775956	-	165	WP_008807599.1	DUF1127 domain-containing protein	-
LGN95_RS23230 (LGN95_23230)	4776134..4777546	+	1413	WP_060619705.1	PLP-dependent aminotransferase family protein	-
LGN95_RS23235 (LGN95_23235)	4777626..4777928	-	303	WP_022065028.1	BrnA antitoxin family protein	Antitoxin
LGN95_RS23240 (LGN95_23240)	4777915..4778202	-	288	WP_012542979.1	BrnT family toxin	Toxin
LGN95_RS23245 (LGN95_23245)	4778370..4778789	-	420	WP_064170638.1	FosA5 family fosfomycin resistance glutathione transferase	-
LGN95_RS23250 (LGN95_23250)	4778783..4779691	-	909	WP_012542980.1	LysR family transcriptional regulator	-
LGN95_RS23255 (LGN95_23255)	4779778..4780560	+	783	WP_012542981.1	NAD(P)H-dependent oxidoreductase	-
LGN95_RS23260 (LGN95_23260)	4780702..4781286	+	585	WP_012542982.1	TetR/AcrR family transcriptional regulator	-
LGN95_RS23265 (LGN95_23265)	4781308..4782111	+	804	WP_012542983.1	winged helix-turn-helix domain-containing protein	-
LGN95_RS23270 (LGN95_23270)	4782108..4782626	+	519	WP_004182811.1	FidL-like protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (38-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11245.77 Da
• Isoelectric Point: 8.6574

>T219324 WP_012542979.1 NZ_CP084766:c4778202-4777915 [Klebsiella variicola subsp. variicola]
MPMEFEWDANKALSNLRKHGVRFEEAVLVFDDPRHLSRQERFENGEYRWQTIGLVHGILVILVAHSVRFESGTEVIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T219324 NZ_CP116285:734123-734338 [Pantoea stewartii]
ATGAACAAAACCCTGACTAAAACTGATTATTTGATGCGACTGAGACGTTGTCGGTCAATTGACACGCTCGAAAGAGTGAT
TGAGAAAAATAAGTATGAATTGTCCGATGATGAATTAGCCGTATTTTACTCCGCAGCCGATCACCGCCTGGCTGAACTGA
CGATGAACAAACTGTATGACAAAGTCCCCGTGTCAGTATGGAAATTTGTACGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11320.98 Da
• Isoelectric Point: 10.3129

>AT219324 WP_022065028.1 NZ_CP084766:c4777928-4777626 [Klebsiella variicola subsp. variicola]
MSMVKHKRGNTAALTAKRDAELKALASKADEDIDYSDIPATDDAQWSDAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Nucleotide

• Length: 303 bp

>AT219324 NZ_CP116285:733721-734098 [Pantoea stewartii]
ATGGACGAATACTCACCGAAACGGCATGATATTGCCCAGCTTAAATACGTGTGTGAGAGTTTGTTTGACGCCGGTATGGC
AACATTAACCGACAGCCATCATGGCTGGGTAAATGACCCTACTGCCGATGGTAATTTACAGCTGAACGATTTGATAGAGC
ATATCGCCTCTTTCACCATGAATTACAAAATTAAGCATGCAGAAGACGAAGCGCTGATTACCCAAATCGATGAATATCTT
GATGATACTTTTATGCTATTTAGCAACTATAGCGTTAACACTCAGGATCTGCAGCGCTGGCAACGTTCAGCAAAACGTTT
ATTTAATCTGTTTCAAGAAGAGTGCGCTTATCTTCAACAGCCAAGCCATTCATTATAG