Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4177018..4177637 Replicon chromosome
Accession NZ_CP084766
Organism Klebsiella variicola subsp. variicola strain 01A065

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LGN95_RS20360 Protein ID WP_002892050.1
Coordinates 4177419..4177637 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag LGN95_RS20355 Protein ID WP_008805436.1
Coordinates 4177018..4177392 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGN95_RS20345 (LGN95_20345) 4172173..4173366 + 1194 WP_008805438.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LGN95_RS20350 (LGN95_20350) 4173389..4176535 + 3147 WP_008805437.1 multidrug efflux RND transporter permease subunit AcrB -
LGN95_RS20355 (LGN95_20355) 4177018..4177392 + 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
LGN95_RS20360 (LGN95_20360) 4177419..4177637 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LGN95_RS20365 (LGN95_20365) 4177796..4178362 + 567 WP_008805435.1 maltose O-acetyltransferase -
LGN95_RS20370 (LGN95_20370) 4178334..4178462 - 129 Protein_3988 hypothetical protein -
LGN95_RS20375 (LGN95_20375) 4178499..4178969 + 471 WP_008805434.1 YlaC family protein -
LGN95_RS20380 (LGN95_20380) 4178938..4180395 - 1458 WP_008805433.1 PLP-dependent aminotransferase family protein -
LGN95_RS20385 (LGN95_20385) 4180496..4181194 + 699 WP_022066288.1 GNAT family protein -
LGN95_RS20390 (LGN95_20390) 4181191..4181331 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LGN95_RS20395 (LGN95_20395) 4181331..4181594 - 264 WP_008805431.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T219323 WP_002892050.1 NZ_CP084766:4177419-4177637 [Klebsiella variicola subsp. variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T219323 NZ_CP116267:c107247-106834 [Aeromonas salmonicida subsp. salmonicida]
GTGAAGACGTTTATGCTCGATACCTGCATTTGTTCCTTCATCATGCGCGAAATGCCACAGTCAGTTCTTGAACGGTTGGG
CGCAGAGGTGGCGAGCGGCAATCGGATTGTGATCTCGGCAATCACCTACGCCGAAATGCGTTATGGCCAGATCGGCAAGA
AGGCTTCTCCGAAGCTGGGGCCAGCCATTGATGAATTTGTTCGTCGTCTCGATGGGATCTTGCCATGGGACGCTGCTACG
GTCGATCAGACGCTCGAAGTGCGCCAGCAGTTAGCAGCCTTGGGCACTCCGATCGGCAATAACGATGCTGCTATTGCCGC
CCACGCCCTGACGGCTGGCTGTGTACTGGTAACCAACAATACCCGCGAGTTTTCCCGAGTGGCGGGGCTGCTCTATGAAG
ACTGGGTTCACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT219323 WP_008805436.1 NZ_CP084766:4177018-4177392 [Klebsiella variicola subsp. variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT219323 NZ_CP116267:c107474-107244 [Aeromonas salmonicida subsp. salmonicida]
ATGCGTACTGTATCAATTTTTAAAAATGGTAAAAATCAGGCGGTGAGACTGCCAAAAGACATGGAGTTTCAGGGGGTTAA
TGACCTTGAGATTATCAAAAATGGCGACACAATCACGTTGCGCCCGGCCCGCCCTGATTGGTTTTCCCTGGCGGAATGCC
AGAAGGTGGATGCAGATTTCTTGCAAGAACGTACCGATGTGATCAGCGATGAAGGACGGTTCGCCCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References