Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1762338..1762928 Replicon chromosome
Accession NZ_CP084766
Organism Klebsiella variicola subsp. variicola strain 01A065

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag LGN95_RS08470 Protein ID WP_008804165.1
Coordinates 1762596..1762928 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1F2LZQ3
Locus tag LGN95_RS08465 Protein ID WP_012541132.1
Coordinates 1762338..1762595 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGN95_RS08445 (LGN95_08445) 1757946..1758521 + 576 WP_032730762.1 hypothetical protein -
LGN95_RS08450 (LGN95_08450) 1758699..1759535 + 837 WP_008804155.1 alpha/beta hydrolase -
LGN95_RS08455 (LGN95_08455) 1759742..1760713 + 972 WP_016161433.1 sensor domain-containing diguanylate cyclase -
LGN95_RS08460 (LGN95_08460) 1760710..1761810 - 1101 WP_022065756.1 AarF/UbiB family protein -
LGN95_RS08465 (LGN95_08465) 1762338..1762595 + 258 WP_012541132.1 antitoxin Antitoxin
LGN95_RS08470 (LGN95_08470) 1762596..1762928 + 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LGN95_RS08485 (LGN95_08485) 1763251..1764687 + 1437 WP_016161429.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
LGN95_RS08495 (LGN95_08495) 1765060..1766514 - 1455 WP_008804170.1 AMP nucleosidase -
LGN95_RS08500 (LGN95_08500) 1766645..1766890 - 246 WP_008804171.1 signal transduction protein PmrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T219312 WP_008804165.1 NZ_CP084766:1762596-1762928 [Klebsiella variicola subsp. variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T219312 NZ_CP116250:5079-5561 [Enterobacter roggenkampii]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTGCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9420.91 Da        Isoelectric Point: 4.4915

>AT219312 WP_012541132.1 NZ_CP084766:1762338-1762595 [Klebsiella variicola subsp. variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT219312 NZ_CP116250:4825-5091 [Enterobacter roggenkampii]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZQ3

References