Detailed information of TA system TA219311

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1577100..1578097
• Replicon: chromosome
• Accession: NZ_CP084766
• Organism: Klebsiella variicola subsp. variicola strain 01A065

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LGN95_RS07675
• Protein ID: WP_229531768.1
• Coordinates: 1577543..1578097 (-)
• Length: 185 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A1F2MDT5
• Locus tag: LGN95_RS07670
• Protein ID: WP_012541033.1
• Coordinates: 1577100..1577546 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGN95_RS07655 (LGN95_07655)	1573395..1574393	+	999	WP_008804028.1	galactose/glucose ABC transporter substrate-binding protein MglB	-
LGN95_RS07660 (LGN95_07660)	1574512..1576032	+	1521	WP_008804029.1	galactose/methyl galactoside ABC transporter ATP-binding protein MglA	-
LGN95_RS07665 (LGN95_07665)	1576048..1577058	+	1011	WP_002912871.1	galactose/methyl galactoside ABC transporter permease MglC	-
LGN95_RS07670 (LGN95_07670)	1577100..1577546	-	447	WP_012541033.1	helix-turn-helix transcriptional regulator	Antitoxin
LGN95_RS07675 (LGN95_07675)	1577543..1578097	-	555	WP_229531768.1	HipA domain-containing protein	Toxin
LGN95_RS07680 (LGN95_07680)	1578126..1578647	-	522	WP_025714688.1	hypothetical protein	-
LGN95_RS07685 (LGN95_07685)	1578762..1579493	-	732	WP_008804033.1	outer membrane permeability protein SanA	-
LGN95_RS07690 (LGN95_07690)	1579530..1579748	-	219	WP_048331011.1	hypothetical protein	-
LGN95_RS07695 (LGN95_07695)	1579770..1580654	-	885	WP_012967554.1	cytidine deaminase	-
LGN95_RS07700 (LGN95_07700)	1580783..1581478	-	696	WP_008804035.1	CidB/LrgB family autolysis modulator	-
LGN95_RS07705 (LGN95_07705)	1581468..1581872	-	405	WP_004201620.1	CidA/LrgA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (2-147)

Antitoxin

• Cro/C1-type helix-turn-helix domain (78-132)

Sequences

Toxin

Protein

• Length: 185 a.a.
• Molecular weight: 20710.03 Da
• Isoelectric Point: 5.7891

>T219311 WP_229531768.1 NZ_CP084766:c1578097-1577543 [Klebsiella variicola subsp. variicola]
MRLGMESVYSLLQKAPATILDHETTLRILIEKITSSNMVRVQNYRFDVQGFIIDWVRRDLLNIIFGNSDNHGRNTAFMKA
DNEIILAPIYDFAPMKADPEGIPRTMKWSLACESGGEYNFGAIAQALAEWITPDTLLNALSETASQLVDLPSRLKVRGVP
VQILEMPSIGFKFIPDKLARWGLL

Nucleotide

• Length: 555 bp

>T219311 NZ_CP116249:4423369-4423746 [Enterobacter roggenkampii]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16396.05 Da
• Isoelectric Point: 10.5417

>AT219311 WP_012541033.1 NZ_CP084766:c1577546-1577100 [Klebsiella variicola subsp. variicola]
MKTLNKKETEIQNTLARLRADNPPSLPATGVTATEKKARSEISRQRVSAGLKKVKTVDRQAVIHAIIHDIMLGAISQGEA
LKKLRVEVLGLRQDEYARLVDVSRKTLSDVENDKGNYSAEIINKIYKPFGLETGLVPISKTLISSLFK

Nucleotide

• Length: 447 bp

>AT219311 NZ_CP116249:4422953-4423279 [Enterobacter roggenkampii]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCTTCGGCTCCTGTGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAA
AAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1F2MDT5