Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5443263..5443888 Replicon chromosome
Accession NZ_CP084765
Organism Klebsiella variicola subsp. variicola strain F2R9T

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag LGN93_RS26295 Protein ID WP_008807903.1
Coordinates 5443263..5443646 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LGN93_RS26300 Protein ID WP_004150355.1
Coordinates 5443646..5443888 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGN93_RS26280 (LGN93_26280) 5440629..5441531 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LGN93_RS26285 (LGN93_26285) 5441528..5442163 + 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
LGN93_RS26290 (LGN93_26290) 5442160..5443089 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LGN93_RS26295 (LGN93_26295) 5443263..5443646 - 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
LGN93_RS26300 (LGN93_26300) 5443646..5443888 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LGN93_RS26305 (LGN93_26305) 5444093..5445010 + 918 WP_023340866.1 alpha/beta hydrolase -
LGN93_RS26310 (LGN93_26310) 5445025..5445966 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
LGN93_RS26315 (LGN93_26315) 5446011..5446448 - 438 WP_008807906.1 D-aminoacyl-tRNA deacylase -
LGN93_RS26320 (LGN93_26320) 5446445..5447305 - 861 WP_008807907.1 virulence factor BrkB family protein -
LGN93_RS26325 (LGN93_26325) 5447299..5447898 - 600 WP_008807908.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T219307 WP_008807903.1 NZ_CP084765:c5443646-5443263 [Klebsiella variicola subsp. variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

>T219307 NZ_CP116249:c3007159-3006638 [Enterobacter roggenkampii]
GTGGACAATCTCACGATCGAAATGTTTTCAGAGGGAAAAGATTATGATTTCAGGGACTTCGATTGTGGTGAACCCTCGCT
AAATGTCTTCCTCACAGAACATCTGGTGCGTCAGCACAATGGGCGTATTTTACGAGGCTATTTGCTTAAAGATCGCGATG
GTCCGCGAGTCCTGGGCTATTACACGTTGTCTGGTAGTTGCTTTGAAAAAGCGATGCTGCCTTCTAAGACTCAGCAGCGT
CGGATCCCTTATAGCAACGTACCGAGCGTAGCGCTGGGACGTCTGGCGATACATAAAGACTTACAGGGGCTGGAGTGGGG
AACGACGCTCGTCACGCATGCCATGCGGGTTGTCTATCTGGCATCGCAGGCGGTGGGCGTACACGGAATATTTGTTGATG
CACTCAACGACCAGGCAAAACAATTTTATCTGAAGCAGGGCTTTATTCCGCTGACGGATGAAAACAGCCATTCTCTTTTC
TTTCCTACAAAATCTATCGAACGACTGTTTGAACAGGCGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT219307 WP_004150355.1 NZ_CP084765:c5443888-5443646 [Klebsiella variicola subsp. variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT219307 NZ_CP116249:c3007435-3007166 [Enterobacter roggenkampii]
ATGCCAGCACTGAAAAAACAGCGTATCGATCTTCGCTTAACGGACGAAGACAAAACAATGATTGAAGAAGCGGCAGCGAT
GACGAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGGGCAGCGGAAGTCATCGAGCAGCACCGCCGTC
TCGTATTAAACGAAGCATCCTGGAATGCCGTGATGGATGCCATCGACAATCCGCCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCGAAACGTCTTCGAAACATGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References