Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4215412..4216031 Replicon chromosome
Accession NZ_CP084765
Organism Klebsiella variicola subsp. variicola strain F2R9T

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LGN93_RS20515 Protein ID WP_002892050.1
Coordinates 4215813..4216031 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag LGN93_RS20510 Protein ID WP_008805436.1
Coordinates 4215412..4215786 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGN93_RS20500 (LGN93_20500) 4210567..4211760 + 1194 WP_040973691.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LGN93_RS20505 (LGN93_20505) 4211783..4214929 + 3147 WP_008805437.1 multidrug efflux RND transporter permease subunit AcrB -
LGN93_RS20510 (LGN93_20510) 4215412..4215786 + 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
LGN93_RS20515 (LGN93_20515) 4215813..4216031 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LGN93_RS20520 (LGN93_20520) 4216190..4216756 + 567 WP_022066286.1 maltose O-acetyltransferase -
LGN93_RS20525 (LGN93_20525) 4216728..4216853 - 126 Protein_4018 hypothetical protein -
LGN93_RS20530 (LGN93_20530) 4216893..4217363 + 471 WP_008805434.1 YlaC family protein -
LGN93_RS20535 (LGN93_20535) 4217332..4218789 - 1458 WP_022066287.1 PLP-dependent aminotransferase family protein -
LGN93_RS20540 (LGN93_20540) 4218890..4219588 + 699 WP_022066288.1 GNAT family protein -
LGN93_RS20545 (LGN93_20545) 4219585..4219725 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LGN93_RS20550 (LGN93_20550) 4219725..4219988 - 264 WP_008805431.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T219305 WP_002892050.1 NZ_CP084765:4215813-4216031 [Klebsiella variicola subsp. variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T219305 NZ_CP116249:1887037-1887303 [Enterobacter roggenkampii]
ATCAGGGACTTGTTCGCACCTTCCCTAAGAAAACATGGAATCCGGTTTGAAGAAGCGGTGCTCGTCTTTGACGATCCCCG
GCATCTTTCCCGACAGGATCGTTATGAGAATGGTGAATATCGCTGGCAAACTCTCAGCCTCGTTCATCGGATCATTGTTA
TCATGGTTGCGCATTCGGTCCGTTTTGAAAGTGGAACAGAGGTCATTCGTATTATCAGCGCCCGGAAGGCGGACAGCAAA
GAGAGAAATCGTTATGAACATGGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT219305 WP_008805436.1 NZ_CP084765:4215412-4215786 [Klebsiella variicola subsp. variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT219305 NZ_CP116249:1887290-1887592 [Enterobacter roggenkampii]
ATGAACATGGTTAAACATAAACGTGACAATCCGGATACGCTTACGACTAAGCGAGACGATGAGCTTAAAGCGCTGGCCAA
CAAAACGGATGATGAAATCGATTATAGCGATATTCCCGCCACAGACGACGCACAGTGGTCAGATGCCGTACGCGGTAAAT
TTTACCGACCGCTAAAAACTCAGGCTTCTGTCCGTATTGATGCCGATATTATGGAGTGGCTTAAACGTCCCGGGAAAGGT
TATCAAACGCGACTGAATGCGATCTTAAGAGAAGCGATGATTCGCGATCTTAACAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References