Detailed information of TA system TA219290

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 328823..329409
• Replicon: chromosome
• Accession: NZ_CP084765
• Organism: Klebsiella variicola subsp. variicola strain F2R9T

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: LGN93_RS01515
• Protein ID: WP_002920800.1
• Coordinates: 329041..329409 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: LGN93_RS01510
• Protein ID: WP_023298302.1
• Coordinates: 328823..329044 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGN93_RS01490 (LGN93_01490)	324972..325898	+	927	WP_012540389.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
LGN93_RS01495 (LGN93_01495)	325895..327172	+	1278	WP_008806971.1	branched chain amino acid ABC transporter permease LivM	-
LGN93_RS01500 (LGN93_01500)	327169..327936	+	768	WP_012967117.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
LGN93_RS01505 (LGN93_01505)	327938..328651	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
LGN93_RS01510 (LGN93_01510)	328823..329044	+	222	WP_023298302.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LGN93_RS01515 (LGN93_01515)	329041..329409	+	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LGN93_RS01520 (LGN93_01520)	329701..331017	+	1317	WP_008806974.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
LGN93_RS01525 (LGN93_01525)	331124..332011	+	888	WP_012967120.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
LGN93_RS01530 (LGN93_01530)	332008..332853	+	846	WP_008806976.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
LGN93_RS01535 (LGN93_01535)	332855..333925	+	1071	WP_022066272.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	325895..334662	8767

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T219290 WP_002920800.1 NZ_CP084765:329041-329409 [Klebsiella variicola subsp. variicola]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T219290 NZ_CP116243:c72890-72612 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATAGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAACCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGAGTATTGCATACTGTACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8123.14 Da
• Isoelectric Point: 4.7769

>AT219290 WP_023298302.1 NZ_CP084765:328823-329044 [Klebsiella variicola subsp. variicola]
MKTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT219290 NZ_CP116243:c73159-72890 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTACTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAAGTCGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1