Detailed information of TA system TA219287

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 4561..5254
• Replicon: plasmid unnamed5
• Accession: NZ_CP084757
• Organism: UNVERIFIED_ORG: Kinneretia sp. XES5

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: LHK14_RS27950
• Protein ID: WP_226923777.1
• Coordinates: 4877..5254 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: LHK14_RS27945
• Protein ID: WP_080921082.1
• Coordinates: 4561..4896 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LHK14_RS27935 (LHK14_27935)	518..1099	-	582	WP_226923796.1	recombinase family protein	-
LHK14_RS27940 (LHK14_27940)	1265..4231	+	2967	WP_226923775.1	Tn3 family transposase	-
LHK14_RS27945 (LHK14_27945)	4561..4896	-	336	WP_080921082.1	helix-turn-helix transcriptional regulator	Antitoxin
LHK14_RS27950 (LHK14_27950)	4877..5254	-	378	WP_226923777.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LHK14_RS27955 (LHK14_27955)	5332..5652	-	321	WP_226923778.1	hypothetical protein	-
LHK14_RS27960 (LHK14_27960)	5649..6308	-	660	WP_226923779.1	AAA family ATPase	-
LHK14_RS27965 (LHK14_27965)	6387..7040	-	654	WP_226923780.1	SprT-like domain-containing protein	-
LHK14_RS27970 (LHK14_27970)	7284..8162	-	879	WP_226923781.1	helix-turn-helix domain-containing protein	-
LHK14_RS27975 (LHK14_27975)	8356..8565	-	210	WP_226923782.1	ribbon-helix-helix protein, CopG family	-
LHK14_RS27980 (LHK14_27980)	8673..9269	-	597	WP_226923784.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..33339	33339
	-	flank	IS/Tn	-	-	518..1138	620
	-	flank	IS/Tn	-	-	8673..9269	596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (18-97)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13934.02 Da
• Isoelectric Point: 9.7698

>T219287 WP_226923777.1 NZ_CP084757:c5254-4877 [Kinneretia sp. XES5]
MNYDERPLEWIGSSYKDLMQLPAGVRRFFGFALNAAQHGEQHTAAKVLKGFGGAGVLELVENDAGGTYRAVYTVRFEEAV
FVLHCFQKKSKSGIATPKADMDVIRARLKVAEIVAKEMRHANPHR

Nucleotide

• Length: 378 bp

>T219287 NZ_CP116241:4464504-4464902 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12141.19 Da
• Isoelectric Point: 9.0961

>AT219287 WP_080921082.1 NZ_CP084757:c4896-4561 [Kinneretia sp. XES5]
MRTRIVNGVEVTTSSGNVFADLGLPDAEKLKMKSGLMIEITKAVRRLDLTQEEAGRRMGLPQPKVSALLRGDFTNLSERK
LMECLNRLGYDIEITVKPAADAVGHMTLAVA

Nucleotide

• Length: 336 bp

>AT219287 NZ_CP116241:4464277-4464504 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA