Detailed information of TA system TA219263

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-higA/HigB-HigA
• Location: 430349..431188
• Replicon: chromosome
• Accession: NZ_CP084741
• Organism: Pectobacterium carotovorum strain A077-S18-O15

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A7V8PT96
• Locus tag: LHL03_RS01965
• Protein ID: WP_039535732.1
• Coordinates: 430826..431188 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LHL03_RS01960
• Protein ID: WP_039470394.1
• Coordinates: 430349..430822 (-)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LHL03_RS01940 (LHL03_01940)	425454..426377	-	924	WP_226509727.1	aminoimidazole riboside kinase	-
LHL03_RS01945 (LHL03_01945)	426701..426889	+	189	WP_039470389.1	hypothetical protein	-
LHL03_RS01950 (LHL03_01950)	427059..427610	+	552	WP_039470391.1	polymer-forming cytoskeletal protein	-
LHL03_RS01955 (LHL03_01955)	427642..430359	+	2718	WP_226509728.1	CRISPR-associated helicase Cas3'	-
LHL03_RS01960 (LHL03_01960)	430349..430822	-	474	WP_039470394.1	helix-turn-helix domain-containing protein	Antitoxin
LHL03_RS01965 (LHL03_01965)	430826..431188	-	363	WP_039535732.1	type II toxin-antitoxin system HigB family toxin	Toxin
LHL03_RS01970 (LHL03_01970)	431802..433370	+	1569	WP_039535734.1	type I-E CRISPR-associated protein Cse1/CasA	-
LHL03_RS01975 (LHL03_01975)	433354..433986	+	633	WP_138255305.1	type I-E CRISPR-associated protein Cse2/CasB	-
LHL03_RS01980 (LHL03_01980)	433990..435057	+	1068	WP_039535739.1	type I-E CRISPR-associated protein Cas7/Cse4/CasC	-
LHL03_RS01985 (LHL03_01985)	435067..435801	+	735	WP_039535741.1	type I-E CRISPR-associated protein Cas5/CasD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (74-111)

Antitoxin

• Antitoxin HigA (43-157)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 14161.38 Da
• Isoelectric Point: 9.7305

>T219263 WP_039535732.1 NZ_CP084741:c431188-430826 [Pectobacterium carotovorum]
MRVISAKAITDAMKKHAKWKVGLKLWLDVFDKPSLRFESYEQLCHVWRNTSCWNVDRIPFALLENESRKGPLDIYVFDIN
GNNCRIIAWLNPNTGSLYVKDVCSHAEYDKWWKAQTKTKR

Nucleotide

• Length: 363 bp

>T219263 NZ_CP116239:c2902573-2902388 [Pseudomonas sp. TUM22785]
ATGAAACACAGCGAATTCAGGCGATGGTTACGGGCCCAGGGGGTCACCTTCGAAGCGGCAAAAGGAAGCCACTTCAAGAT
CACAGCCCCCAACGGCAAGACGACGATCTTCGCGGATCACGGCTCCAAGGAAATGAAAGAGCCGACCCGCAAGGCAATCA
TCAAGCAACTGGGGCTCACCGAGTGA

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 17763.02 Da
• Isoelectric Point: 4.2043

>AT219263 WP_039470394.1 NZ_CP084741:c430822-430349 [Pectobacterium carotovorum]
MRNTSLGSNSSVGISGLIENLPLLLQEASEQLNSLSVLLSACMEPIEDDNDLQERMECINQLYLYSSDVNDIPATFAELI
ADRVYEYEKKHRDVPHVSQAEALAFFIEERGLKQSDLKQIATQSVISDIINGKRTMTLQQVKDFSRYFNVPVETFID

Nucleotide

• Length: 474 bp

>AT219263 NZ_CP116239:c2902337-2901894 [Pseudomonas sp. TUM22785]
ATGTTCGACTATCCAATCCGGCTTGAAGCCGATGACACTCCAGGGCTGGCAGTCTTCTGCCGCGATCTTCCAGAGCTGAA
CAGCTACGGCGATGACGTTGCGCATGCGCTGGCCGAAGCCGTGGACGCCATCGAGTCCACCCTCTCCCTCTACGTGGACC
AGCGCCGCGCTATTCCTGCTGCGAGCCCTGCTCAGGCTGGCGAGCATGCGGTGCGGCTACCCGCCGTCACCGTCGCCAAG
ATCTACCTCTGGAACGCCATGATGGAGCAGGGTCTGCGCAAGGCAGACCTATGCCGCCTGCTAGGCGTCGCGCCCATGCA
GGTCGACCGCTTGGTGGACTTCCTCCACACCTCGAAGATGGAGGCAGTCGAAGCGGCATTGGCGAAGCTTGGTAAGCGGC
TAGCGGTAAGTGTTGAAGCTGGAGAGTGGCCGCAAAGCGCATGA

Structures

Toxin

• AlphaFold DB: A0A7V8PT96