Detailed information of TA system TA219250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1492583..1493109
• Replicon: chromosome
• Accession: NZ_CP084740
• Organism: Dickeya zeae strain A586-S18-A17

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: LHK94_RS06675
• Protein ID: WP_110371300.1
• Coordinates: 1492804..1493109 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A2K8W1V7
• Locus tag: LHK94_RS06670
• Protein ID: WP_022632052.1
• Coordinates: 1492583..1492801 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LHK94_RS06635 (LHK94_06640)	1487814..1488389	+	576	WP_226511633.1	transcriptional regulator	-
LHK94_RS06645 (LHK94_06650)	1488788..1490047	+	1260	WP_226511303.1	integrase arm-type DNA-binding domain-containing protein	-
LHK94_RS06650 (LHK94_06655)	1490153..1490347	-	195	WP_226511304.1	YlcI/YnfO family protein	-
LHK94_RS06655 (LHK94_06660)	1490534..1490884	+	351	WP_013316250.1	hypothetical protein	-
LHK94_RS06660 (LHK94_06665)	1491035..1491208	-	174	WP_226511305.1	hypothetical protein	-
LHK94_RS06665 (LHK94_06670)	1491781..1492524	+	744	WP_110371301.1	MobC family replication-relaxation protein	-
LHK94_RS06670 (LHK94_06675)	1492583..1492801	+	219	WP_022632052.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
LHK94_RS06675 (LHK94_06680)	1492804..1493109	+	306	WP_110371300.1	type II toxin-antitoxin system toxin CcdB	Toxin
LHK94_RS06680 (LHK94_06685)	1493506..1495833	+	2328	WP_226511306.1	TerB N-terminal domain-containing protein	-
LHK94_RS06685 (LHK94_06690)	1495837..1497153	+	1317	WP_226511307.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1488611..1508435	19824

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11601.52 Da
• Isoelectric Point: 5.6015

>T219250 WP_110371300.1 NZ_CP084740:1492804-1493109 [Dickeya zeae]
MQFIVYQYKRASHYKMFVDVQSDIVETPKRRMVIPLIEAHHLSEKVNKTLFPLIRIDGEDYRLMTTELSSVPVDVIGGAL
VDLGGYADEIKDAINLMFWGI

Nucleotide

• Length: 306 bp

>T219250 NZ_CP116231:1982787-1983047 [Fusobacterium nucleatum]
ATGAATAATTTAGTATGGACACATAAGGCTTGGCAAGATTACCTATATTGGCAAACTCAGGATAAAAAAACTTTAAAAAA
GATAAATGAATTAGTCAAAGATATTGAAAGAAATGGAGCTTTAAATGGGATAGGCAAACCAGAAGTATTAAAAAATGAAA
GTGCATATAGTAGAAGAATTGATGAAAAAAATAGACTGGTTTATAGAATTGTAGATGGTTTTATTTGGATAATTGCTTGT
AAAGGTCATTATGAGGAATAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8109.02 Da
• Isoelectric Point: 5.8316

>AT219250 WP_022632052.1 NZ_CP084740:1492583-1492801 [Dickeya zeae]
MKHRVSVTVDKDNYQVLSAAGVNISGLVNDAIGKEARRIKAEEWKKENSEGMEEVARFIAQNGSFADENRNW

Nucleotide

• Length: 219 bp

>AT219250 NZ_CP116231:1982540-1982794 [Fusobacterium nucleatum]
ATGTTAGCAATTAATTATACAACTTTGAGAACCAATTTAAAAAGCTATTTTGATAAAGCAGTAGATAATGATGAAGATAT
TATTATAACAAGAAAAAATGAAAGAAATGTTGTTCTATTAAGTCTTGATAAATATAATGAGTTTTTAAAAGCTATGAGAA
ATCTTGAATATATGACAAAAATAAGAGAAGGAATAGCTGAATTAGAGGCAGGAAAAGGAGAAATTCATGATTTAATAGAG
GTTGATGATGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A2K8W1V7