Detailed information of TA system TA219247

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DhiAT/dnstrm_HI1420(antitoxin)
• Location: 481333..482083
• Replicon: chromosome
• Accession: NZ_CP084740
• Organism: Dickeya zeae strain A586-S18-A17

Toxin (Protein)

• Gene name: dhiT
• Uniprot ID: -
• Locus tag: LHK94_RS02215
• Protein ID: WP_210182173.1
• Coordinates: 481826..482083 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: dhiA
• Uniprot ID: D2C389
• Locus tag: LHK94_RS02210
• Protein ID: WP_012885282.1
• Coordinates: 481333..481818 (-)
• Length: 162 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LHK94_RS02185 (LHK94_02190)	477267..478994	+	1728	WP_168362930.1	hydrogenase large subunit	-
LHK94_RS02190 (LHK94_02195)	479005..479574	+	570	WP_016943631.1	hydrogenase 4 subunit H	-
LHK94_RS02195 (LHK94_02200)	479571..480359	+	789	WP_210182174.1	NADH-quinone oxidoreductase subunit B family protein	-
LHK94_RS02200 (LHK94_02205)	480359..480757	+	399	WP_016943629.1	formate hydrogenlyase maturation HycH family protein	-
LHK94_RS02205 (LHK94_02210)	480750..481244	+	495	WP_080650191.1	hydrogenase maturation peptidase HycI	-
LHK94_RS02210 (LHK94_02215)	481333..481818	-	486	WP_012885282.1	DUF2442 domain-containing protein	Antitoxin
LHK94_RS02215 (LHK94_02220)	481826..482083	-	258	WP_210182173.1	DUF4160 domain-containing protein	Toxin
LHK94_RS02220 (LHK94_02225)	482618..483160	+	543	WP_016943624.1	4Fe-4S dicluster domain-containing protein	-
LHK94_RS02225 (LHK94_02230)	483195..485345	+	2151	WP_210182172.1	formate dehydrogenase subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF4160 (9-73)

Antitoxin

• Protein of unknown function DUF2442 (4-70)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9878.48 Da
• Isoelectric Point: 7.5161

>T219247 WP_210182173.1 NZ_CP084740:c482083-481826 [Dickeya zeae]
MPEIDRLAGLIFYLYFYDDERHHLPHVHVCYGNHELIISIRTGAEIEGYLPNNKRKLAIAHIMTHREQLLSMWAQAVIGK
NPGKL

Nucleotide

• Length: 258 bp

>T219247 NZ_CP116225:c301844-301554 [Actinomyces oris]
GTGAGCACCTACCGTCTGACCCCGGCTGCGCGCCGCGATCTGTCACGTATCTGGGACTACAGCGAAGAGCGCTGGGGGCT
GCGGCAGGCCGAGGTCTATCTGCGTGATCTTCAGACGTGTCTGGAAAGACTCGCAGACGATCCTCGTCGAGGGCATCCCA
GAGACGAGGTACGTCCCGGCTACCGAAGCCGCGCCGTCGGAAGCCATGTCGTCTTCTACGTCATCAGTGATGGTGGCGTT
GACGTGATCCGTGTGCTCCACCAACGCATGGACCCCGACAGGCACGTGTAG

Antitoxin

Protein

• Length: 162 a.a.
• Molecular weight: 17784.21 Da
• Isoelectric Point: 6.4900

>AT219247 WP_012885282.1 NZ_CP084740:c481818-481333 [Dickeya zeae]
MHKIVDVDVIGDFSLRLTYDDGLICDVNLYHYASHPVFSNASLFVRFGLLPDGSLEWPDITISSSVLREKGENVGHTLAV
RERNFADIISRAFYDAVIENRPEILQAALKIAVDKHGNSTVAKEAGAKSRTSLYKSLNKNTNVSLATVVSLAHTVLQLEE
K

Nucleotide

• Length: 486 bp

>AT219247 NZ_CP116225:c302083-301841 [Actinomyces oris]
ATGTCAGTGAACACCTCCGTCAGCCTCGATGACCACTTCGTCGGATTCATCGCGAGCCAAGTCGGGTCCGGACGGTACCG
GTCCGCGAGCGAGGTCATCCGCGCCGGCCTGCGCCTTCTGGAGGACCAGGAGACAGAGATGTCGGCGCTGCGCGAGGCAC
TCGCATCCGGTGAGGCGAGCGGACAGGCGGAGCCCTTCGACTTCGACGCCTTCATCGCCGCCAAGGCGGCAAGGACGTCG
TGA

Structures

Antitoxin

• AlphaFold DB: D2C389